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Complete Genome Sequence of Pedobacter sp. PAMC26386 and Their Low Temperature Application in Arabinose-containing Polysaccharides Degradation.

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Department of Life Science and Biochemical Engineering, Graduate School, Sunmoon University, 70 Sunmoonro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Korea.

Pedobacter are a representative genus of soil-associated bacteria. Here we have provided the complete genome sequence of Pedobacter sp. PAMC26386 isolated from Antarctic soil, and functionally annotated the genome, describing the unique features of carbohydrate active enzymes (CAZymes) and α-L-arabinofuranosidase (α-L-ABF).

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November 2019

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Article Synopsis
  • The study identified and cloned two new genes encoding α-l-arabinofuranosidases (ABFs) from Penicillium chrysogenum 31B, adding to the previously known four enzymes.
  • Both enzymes, PcABF43B and PcABF51C, displayed distinct optimal activity conditions, with PcABF43B functioning best at low temperatures (20°C) and PcABF51C at higher temperatures (45°C).
  • The substrate specificity analysis revealed that PcABF43B preferentially hydrolyzed branched arabino-oligosaccharides, while PcABF51C and another enzyme (AFQ1) showed more efficiency on linear forms, indicating their different roles in degrading dietary fibers.
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Switchgrass (Panicum virgatum, L.) is a potential renewable source of carbohydrates for use in microbial conversion to biofuels. Xylan comprises approximately 30% of the switchgrass cell wall.

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