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An unusual case of reagent interference in transfusion medicine workup: Pan reactive indirect antiglobulin test caused by commercial saline solution.
Transfus Med
November 2024
Department of Clinical Immunology, Odense University Hospital, Odense, Denmark.
Background: At the regional transfusion service in the Region of Southern Denmark, serological investigations are primarily carried out using column agglutination techniques. This case study examines an unusual instance of reagent interference in pretransfusion testing using column agglutination at the Hospital of Southern Denmark, Aabenraa.
Case Presentation: A 72-year-old male patient presented for pretransfusion testing prior to hernia surgery.
Evaluation of the analytical performance of four different manufacturer's reagent red blood cells in antibody detection and identification.
BMC Res Notes
October 2024
School of Health and Biomedical Sciences, STEM College, RMIT University, PO Box 71, Bundoora, VIC, 3083, Australia.
Objective: The detection/identification of clinically significant antibodies to red cell antigens form the foundation for safe transfusion practices. This study aimed to evaluate the diagnostic performance of commercially available 0.8% reagent red blood cells (RRBCs) in Australia.
View Article and Find Full Text PDFMethods and applications of noncompetitive hapten immunoassays.
Anal Methods
August 2024
Shenzhen New Industries Biomedical Engineering Co., Ltd., Shenzhen 518118, China.
Hapten immunoassays have found extensive application across various domains such as disease diagnostics, environmental monitoring, as well as the evaluation of food and pharmaceutical safety. These techniques traditionally rely on competitive assay formats and often face challenges with sensitivity and specificity. This review focuses on the emergent noncompetitive immunoassay technologies that promise to transcend these limitations through innovative approaches.
View Article and Find Full Text PDFBelimumab concentration measurements using a homologous anti-idiotype immunoassay.
J Immunol Methods
September 2024
Biologics Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands; Amsterdam Institute for Infection and Immunity, Amsterdam, the Netherlands; Department of Immunopathology, Sanquin Research, Amsterdam, The Netherlands, and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands. Electronic address:
Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest.
View Article and Find Full Text PDFA national survey of current immunohematologic testing practices for the diagnosis of autoimmune hemolytic anemia in India.
Immunohematology
June 2024
6Department of Transfusion Medicine, Tata Medical Center, Rajarhat, New Town, Kolkata India.
Autoimmune hemolytic anemia (AIHA) is a common term for several disorders that differ from one another in terms of etiology, pathogenesis, clinical features, and treatment. Management of patients with AIHA has become increasingly evidence-based in recent years. While this development has resulted in therapeutic improvements, it also carries increased requirements for optimal diagnosis using more advanced laboratory tests.
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