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A solid-phase enzyme-linked immunoassay for the detection of hepatitis B e-antigen (HBeAg) and antibody (anti-HBe) was developed and compared with rheophoresis and radioimmunoassay (RIA). The enzyme-immunoassay (EIA) was similar to RIA in sensitivity and was approximately 1000-fold more sensitive than rheophoresis for HBeAg, and approximately 6000-fold more sensitive than rheophoresis for anti-HBe.
View Article and Find Full Text PDF[Detection of hepatitis Be antigen with an enzyme immunoassay (author's transl)].
Zentralbl Bakteriol A
August 1980
An enzyme immunoassay (ELISA) from SEROMED was tested for its suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis. From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.
View Article and Find Full Text PDFBlood samples from 154 asymptomatic carriers of HBsAg were studied for the presence of HbeAg and anti-HBe using techniques of rheophoresis and a micro solid phase radioimmunoassay (micro-SPRIA). The level of HBsAg in each sample was determined by titration using reverse passive hemagglutination (RPHA) test. The significance of relationship between the titer of HBsAg, HBeAg, anti-HBe, and anti-HBc were statistically analyzed.
View Article and Find Full Text PDFUsing ultracentrifugation, gel filtration, and ion exchange, an attempt was made to completely separate immunoglobulin IgM and IgG present in pooled human serum of blood group O. The quality of separation was studied by testing IgG fractions for the presence of IgM by a sensitive detection method. It is demonstrated by rheophoresis that small amounts of IgM always remain detectable in the IgG fraction.
View Article and Find Full Text PDFA total of 3,212 samples of blood from asymptomatic volunteer donors positive for hepatitis B surface antigen (HBsAg) by radioimmunoassay and 860 samples negative for HBsAg were tested for the presence of hepatitis e antigen (HBeAg) and its corresponding antibody (anti-HBe) using a rheophoresis technique. Neither HBeAg nor anti-HBe was detected in any sample negative for HBsAg. Of the HBsAg-positive samples, 237 (7.
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