Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Analysis of α-glucosidase enzyme activity used in a rapid test for steam sterilization assurance.
J Appl Microbiol
May 2016
Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, USA.
Aims: This study was to determine the sources, location and identity of α-glucosidases in dormant/germinating/outgrowing spores and growing cells of Geobacillus stearothermophilus ATCC 7953, an enzymatic activity in spores used in rapid tests of steam sterilization.
Methods And Results: α-Glucosidase activity in spores and cells was determined measuring methylumbelliferyl-α-d-glucoside (α-MUG) or α-MUG-6-phosphate hydrolysis fluorometrically. While α-MUG-6-phosphate was not hydrolysed by cell or spore extracts, assays with α-MUG showed that: (1) the α-glucosidase activity was inside and outside spores, and the activity outside spores was largely removed by buffer washes or heat activation, whereas α-glucosidase activity was only inside vegetative cells; (2) most α-glucosidase activity in cells and spores was soluble; (3) Western blots and enzyme inhibition using an anti-α-glucosidase antiserum identified ≥2 α-glucosidases in spores and growing cells; (4) α-glucosidase-specific activities were similar in dormant, germinated and outgrowing spore and growing cell extracts; and (5) significant α-glucosidase was synthesized during spore germination and outgrowth and cell growth, this synthesis was not repressed by glucose nor induced by α-MUG, but glucose inhibited α-MUG uptake.
Alpha-glucosidase in the human epididymis: topographic distribution and clinical application.
Andrologia
October 2004
Departments of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Box 54-D, Temuco, Chile.
Alpha-glucosidase activity (EC.3.2.
View Article and Find Full Text PDFA cytosolic glucosyltransferase is required for conversion of starch to sucrose in Arabidopsis leaves at night.
Plant J
March 2004
Department of Metabolic Biology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Maltose is exported from the Arabidopsis chloroplast as the main product of starch degradation at night. To investigate its fate in the cytosol, we characterised plants with mutations in a gene encoding a putative glucanotransferase (disproportionating enzyme; DPE2), a protein similar to the maltase Q (MalQ) gene product involved in maltose metabolism in bacteria. Use of a DPE2 antiserum revealed that the DPE2 protein is cytosolic.
View Article and Find Full Text PDFcDNA cloning and functional expression of alpha-glucosidase from Mortierella alliacea.
Appl Microbiol Biotechnol
August 2003
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, 739-8530, Higashi-Hiroshima, Japan.
We recently purified an alpha-glucosidase comprising 61-kDa and 31-kDa subunits from the fungus Mortierella alliacea and characterized its soluble starch-hydrolyzing activity. Here, the cDNA coding for this enzyme was cloned, revealing that it encodes a single polypeptide of 1,053 amino acids, with a calculated molecular mass of 117 kDa. Comparison between the deduced amino acid sequence and the partial sequences of the purified enzyme suggested that an immature protein can be converted into the two subunits of mature enzyme by post-translational processing at least three cleavage sites.
View Article and Find Full Text PDFCharacterization of the stunting syndrome agent: relatedness to known viruses.
Avian Dis
June 2000
Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
An enteric disease of young turkeys, referred to as stunting syndrome (SS), causes reduced growth and impaired feed efficiency. A recently isolated virus, stunting syndrome agent, (SSA) has been found to be the etiologic agent of SS. The objective of the present study was to determine relatedness of the SSA with other viral agents.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!