From the effects of N-acetyl-d-glucosamine and d-glucose on the elution of the A protein of human lactose synthetase from a column of Sepharose-alpha-lactalbumin, values of 200m(-1) and 0.57m(-1) are deduced for the association constants describing the interaction between the enzyme and the respective monosaccharides.
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http://dx.doi.org/10.1042/bj1350897 | DOI Listing |
Anal Sci
December 2024
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan.
A biosensor for biochemical oxygen demand (BOD) was developed based on intracellular 5'-adenosine triphosphate (ATP) measurements in Saccharomyces cerevisiae. Intracellular ATP was measured using an engineered protein named ATeam, comprising a bacterial FF-ATP synthase ε subunit sandwiched between cyan fluorescent protein and mVenus, a modified yellow fluorescent protein. Because the binding of ATP to ATeam induces changes in the fluorescence spectra owing to Fӧrster resonance energy transfer, S.
View Article and Find Full Text PDFNewborn (Clarksville)
March 2024
Global Newborn Society, Clarksville Maryland, United States of America.
Microb Cell Fact
October 2024
State Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Appl Environ Microbiol
October 2024
Department of Food Science, University of Wisconsin-Madison, Madison, Wisconsin, USA.
J Dairy Sci
December 2024
Division of Animal Sciences, University of Missouri, Columbia, MO 65211. Electronic address:
Increased transcription of genes involved in lactose synthesis is a key feature of secretory activation of the mammary gland. We determined which genes are transcriptionally regulated during the transition from colostrum to milk in cows and whether more frequent milking of one udder half would increase milk yields and alter gene expression. We enrolled 12 Holstein cows immediately after calving, harvested colostrum, then randomly assigned one udder half to 12-h (2X) or 6-h (4X) milking intervals for 48 h after first milking.
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