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Mapping the primate thalamus: systematic approach to analyze the distribution of subcortical neuromodulatory afferents.
Brain Struct Funct
June 2023
Department of Anatomy, Histology and Neuroscience, School of Medicine, Facultad de Medicina, Universidad Autónoma de Madrid, Calle Arzobispo Morcillo 4, 28029, Madrid, Spain.
Neuromodulatory afferents to thalamic nuclei are key for information transmission and thus play critical roles in sensory, motor, and limbic processes. Over the course of the last decades, diverse attempts have been made to map and describe subcortical neuromodulatory afferents to the primate thalamus, including axons using acetylcholine, serotonin, dopamine, noradrenaline, adrenaline, and histamine. Our group has been actively involved in this endeavor.
View Article and Find Full Text PDFAnaesthetic management of a patient with eosinophilic granulomatosis with polyangiitis for internal fixation of a fractured femur.
BMJ Case Rep
January 2021
Department of Anaesthesia and Intensive Care, Cork University Hospital, Cork, Ireland.
Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease with an estimated annual incidence of 0.5-6.8 per million.
View Article and Find Full Text PDFPneumococcus Infection of Primary Human Endothelial Cells in Constant Flow.
J Vis Exp
October 2019
Institut für Mikrobiologie, Technische Universität Braunschweig;
Interaction of Streptococcus pneumoniae with the surface of endothelial cells is mediated in blood flow via mechanosensitive proteins such as the Von Willebrand Factor (VWF). This glycoprotein changes its molecular conformation in response to shear stress, thereby exposing binding sites for a broad spectrum of host-ligand interactions. In general, culturing of primary endothelial cells under a defined shear flow is known to promote the specific cellular differentiation and the formation of a stable and tightly linked endothelial layer resembling the physiology of the inner lining of a blood vessel.
View Article and Find Full Text PDFImmunohistochemical Approach to Understanding the Organization of the Olfactory System in the Cuttlefish, Sepia officinalis.
ACS Chem Neurosci
August 2018
Sorbonne Université, MNHN, UNICAEN, UA, CNRS, IRD, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Paris 75005 , France.
Cephalopods are nontraditional but captivating models of invertebrate neurobiology, particularly in evolutionary comparisons. Cephalopod olfactory systems have striking similarities and fundamental differences with vertebrates, arthropods, and gastropods, raising questions about the ancestral origins of those systems. We describe here the organization and development of the olfactory system of the common cuttlefish, Sepia officinalis, using immunohistochemistry and in situ hybridization.
View Article and Find Full Text PDFImproved cell membrane bioaffinity sample pretreatment technique with enhanced stability for screening of potential allergenic components from traditional Chinese medicine injections.
J Mater Chem B
January 2018
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
Aiming at improving reliability and tedious analysis time in conventional cell membrane chromatography, an improved bioaffinity sample pretreatment technique with enhanced stability was developed to fast screen and extract potential allergenic components from traditional Chinese medicine injections. In this study, rat basophilic leukemia-2H3 cell membrane coated silica particles (RBL-2H3/CMCSPs) were fabricated by irreversible adsorption between the cell membrane and silica and self-fusion of the cell membrane, which could simulate drug-receptor interactions in vitro. Also, benefiting from the use of paraformaldehyde, the average recoveries of the six batches of RBL/CMCSPs were 90.
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