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Simultaneous determination of naphthalimide-labelled monosaccharides in . Hua. polysaccharides utilizing the HPLC-UV technique.
Anal Methods
January 2025
Key Laboratory of Xin'an Medicine of the Ministry of Education, College of Chinese Medicine, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230038, P. R. China.
4-Amidogen-1,8-naphthalimide (ANA), a novel pre-column derivatization reagent, has been successfully synthesized and utilized for the highly sensitive analysis of monosaccharides. ANA reacts with the reducing carbonyl groups of saccharides, facilitating monosaccharide detection. The resulting monosaccharide derivatives were meticulously investigated using High-Performance Liquid Chromatography (HPLC) coupled with ultraviolet (UV) spectroscopy.
View Article and Find Full Text PDFStructural analysis and anti-hepatic fibrosis effects of a homogeneous polysaccharide from Radix Puerariae lobatae (Willd.) Ohwi roots.
Int J Biol Macromol
January 2025
Center for Xin'an Medicine and Modernization of Traditional Chinese Medicine of IHM, Wannan Medical College, Wuhu 241002, China; Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui, Wannan Medical College, Wuhu 241002, China; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wannan Medical College, Wuhu 241002, China; Anhui Innovative Center for Drug Basic Research of Metabolic Diseases, Wannan Medical College, Wuhu 241002, China; Wuhu Modern Technology Research and Development Center of Chinese herbal Medicines and Functional Foods, Anhui College of Traditional Chinese Medicine, Wuhu 241002, China. Electronic address:
Radix Pueraria lobata (Willd.) Ohwi, renowned for its medicinal properties, has garnered significant research interest, particularly in its polysaccharide components. In this study, a novel water-soluble polysaccharide (50PLP) was isolated and characterized from P.
View Article and Find Full Text PDFDiscovery and mechanistic exploration of promiscuous xylosyltransferase based on protein engineering.
Int J Biol Macromol
January 2025
National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing 100700, China.
Glycosylation is an effective means to alter the structure and properties of plant compounds, influencing the pharmacological activity of natural products (NPs) to obtain highly active NPs. In nature, glucosides are the most widely distributed, while other glycosides such as xylosides are less common and present in lower quantities. This is due to the scarcity of xylosyltransferases with substrate promiscuity in nature, and the modification of their catalytic function is also quite challenging.
View Article and Find Full Text PDFUnusual free trisaccharides in caprine colostrum discovered by logically derived sequence tandem mass spectrometry.
Sci Rep
January 2025
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106216, Taiwan.
Free oligosaccharides in human milk have many biological functions for infant health. The reducing end of most human milk oligosaccharides is lactose, and caprine milk was reported to contain oligosaccharides structurally similar to those present in human milk. The structures of oligosaccharides were traditionally determined using nuclear magnetic resonance spectroscopy or enzyme digestion followed by various detection methods, e.
View Article and Find Full Text PDFTemperature Regulates Astroglia Morphogenesis Through Thermosensory Circuitry in Caenorhabditis elegans.
Glia
January 2025
State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science and Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, P. R. China.
Astrocytes are the most abundant type of macroglia in the brain and play crucial roles in regulating neural development and functions. The diverse functions of astrocytes are largely determined by their morphology, which is regulated by genetic and environmental factors. However, whether and how the astrocyte morphology is affected by temperature remains largely unknown.
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