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Optimization of the arginase activity assay micromethod for macrophages and sera.
BMC Res Notes
August 2023
Laboratoire de Parasitologie, UMR 177 IRD/CIRAD "INTERTRYP, " Université Bordeaux, Bordeaux, F-33000, France.
Objective: We optimized the spectrophotometric micromethod for the determination of arginase activity based on the Corraliza et al. modification of Schimke's method. Arginase activity in sera from patients suffering from human African trypanosomiasis, in macrophage lysates from trypanosome-infected mice, and in purified bovine liver arginase was compared using the conventional and optimized micromethods.
View Article and Find Full Text PDFStudy the impact of astaxanthin on developing of genomic instability in human peripheral blood lymphocytes irradiated in vitro on G2 phase of cell cycle.
Probl Radiac Med Radiobiol
December 2017
State institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», Melnykova str., 53, Kyiv, 04050, Ukraine.
Objective: To identify the possibility of modification by astaxanthin the level of genome damages induced by gamma quanta in the culture of human peripheral blood lymphocytes exposed in vitro on postsynthetic (G2) phase of the first mitotic cycle.
Materials And Methods: Peripheral blood lymphocytes from four apparently healthy volunteers 35-51 years old were cultivated using modified micromethod. To obtain genomic damages in G2 phase of the first mitotic cycle the part of cultures was irradiated by γ quanta in dose 1.
Gas chromatography-mass spectrometry microanalysis of alpha- and gamma-tocopherol in plasma and whole blood.
Clin Chim Acta
June 2015
Department of Medico-Surgical Sciences and Biotechnologies, Laboratory of Vascular Biology and Mass Spectrometry, Sapienza University of Rome, Latina, Italy. Electronic address:
Background: Assessing vitamin E status in humans is critical for nutritional evaluation and verification of clinical and biological compliance of supplemented subjects. An accurate analytical method for measuring the two main vitamin E isoforms, i.e.
View Article and Find Full Text PDFTo examine the state of the biliary system, a study of properties of bile "case-control") 100 children and adolescents aged 8 to 18 years, held checkup in consultative and diagnostic center for chronic gastroduodenitis. BMI children were divided into 2 groups: group 1-60 children with obesity (BMI of 30 to 40) and group 2-40 children with normal anthropometric indices. Survey methods included clinical examination pediatrician, endocrinologist, biochemical parameters (ALT, AST, alkaline phosphatase level, total protein, bilirubin, lipidogram, glucose, insulin, HOMA-index), ultrasound of the abdomen and retroperitoneum, EGD with aspiration of gallbladder bile.
View Article and Find Full Text PDFEvaluation of the performance of a micromethod for measuring urinary iodine by using six sigma quality metrics.
Ann Lab Med
September 2013
Cardiovascular, Diabetes & Nutrition Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia.
Background: The urinary iodine micromethod (UIMM) is a modification of the conventional method and its performance needs evaluation.
Methods: UIMM performance was evaluated using the method validation and 2008 Iodine Deficiency Disorders survey data obtained from four urinary iodine (UI) laboratories. Method acceptability tests and Sigma quality metrics were determined using total allowable errors (TEas) set by two external quality assurance (EQA) providers.
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