Chloramphenicol-specific antibody. 3. Differential antibody decay rates to separate determinants on one antigen.

In rabbits, peak titres to repeated immunization with chloramphenicol bound to bovine γ-globulin (CAP—BGG) appear on the 4th to 7th day for the BGG part of the antigen, but not until the 9th to 12th day for CAP. Similarly the antibody to CAP declines in titre much more rapidly than the antibody to the BGG and 4–6 weeks later anti-CAP antibody is no longer detectable while the anti-BGG antibody is still found in high titre.