We found reduced values for formaldehyde-treated influenza virus in the single-radial-diffusion test by comparison with non-treated virus. The reduction correlates with the formaldehyde concentration. Hemagglutination and neuraminidase activities were not affected by the same treatment. We therefore suggest that this reduction is not based on antigen destruction.
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Approaches to the control of acellular pertussis vaccines.
Biologicals
June 1999
Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, U.K.
The quality control of acellular pertussis vaccines presents particular problems related to the differences in composition and method of detoxification used in the various type of preparation. These vaccines are not amenable to potency assay by the active mouse protection test used for whole-cell pertussis vaccines and assurance of protective activity is problematic. In contrast, monitoring of these vaccines for safety is relatively straightforward and is centred on assays for the lipooligosaccharide endotoxin, active pertussis toxin and absence of reversion to toxicity of detoxified product.
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View Article and Find Full Text PDFThe isotypic, allotypic and idiotypic heterogeneity of bovine IgG2.
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December 1987
Department of Microbiology, University of Iowa Medical School, Iowa City 52242.
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View Article and Find Full Text PDFThe identification tests for adsorbed diphtheria, tetanus and pertussis vaccines, which are required by the European Pharmacopoeia to be undertaken in animals, may be replaced by precipitation tests, for instance in agaros gels. Such in vitro tests eliminate the use of animals and are less expensive and time-consuming. The single radial immunodiffusion technique is a suitable semiquantitative test, while the double diffusion test is necessary for the investigation of complete or partial identity.
View Article and Find Full Text PDFArticle Synopsis
- Researchers investigated poliovirus reactions in single-radial-immuno diffusion (SRD) tests to improve antigen detection systems.
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