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Rapid Tryptophan Assay as a Screening Procedure for Quality Protein Maize.
Molecules
September 2024
Integrated Centre of Environmental Science Studies in the North East Region-CERNESIM Centre, Institute of Interdisciplinary Research, Alexandru Ioan Cuza University of Iasi, 11 Carol I, 700506 Iasi, Romania.
Tryptophan is an essential amino acid deficient in cereals, especially maize. However, maize ( L.) is the main source of protein in some developing countries in Africa and Latin America.
View Article and Find Full Text PDFPolyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development.
Rev Paul Pediatr
March 2024
Universidad Nacional de Córdoba, Facultad de Ciencias Médicas, Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Ciudad de Córdoba, Córdoba, Argentina.
Objective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes.
Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p<0.
Evaluation of a microscale quantitative suspension test to determine the bactericidal and yeasticidal activity of glutaral - one step to improve sustainability in disinfectant testing.
GMS Hyg Infect Control
January 2024
Institute for Hygiene and Public Health, University Clinics Bonn, Bonn, Germany.
Aims: To evaluate a newly developed microscale quantitative suspension test compared to the existing standard suspension test using determination of the bactericidal and yeasticidal activity of glutaral as one step to improve the sustainability of disinfectant testing.
Methods: The testing principles of the quantitative suspension test according to VAH method 9 (comparable to EN 13727) was used as a standard suspension test using 8.0 mL product test solution, 1.
Whole blood no-lyse no-wash micromethod for the quantitative measurement of monocyte HLA-DR.
Cytometry B Clin Cytom
January 2024
Institute of Integrative Biology of the Cell, CNRS, CEA, Paris-Saclay University, Gif-sur-Yvette, France.
Background: Monocyte (m)HLA-DR expression appears to be a potent marker of immunosuppression in critically ill patients. The persistence of low mHLA-DR expression is associated with an increased risk of nosocomial infections and mortality. To adapt this measurement to pediatric requirements and provide extensive 24/7 access, we have developed a whole blood no-lyse no-wash micromethod (MM) and compared it with the standardized method (SM).
View Article and Find Full Text PDFOptimization of the arginase activity assay micromethod for macrophages and sera.
BMC Res Notes
August 2023
Laboratoire de Parasitologie, UMR 177 IRD/CIRAD "INTERTRYP, " Université Bordeaux, Bordeaux, F-33000, France.
Objective: We optimized the spectrophotometric micromethod for the determination of arginase activity based on the Corraliza et al. modification of Schimke's method. Arginase activity in sera from patients suffering from human African trypanosomiasis, in macrophage lysates from trypanosome-infected mice, and in purified bovine liver arginase was compared using the conventional and optimized micromethods.
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