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Prevalence and herd-level risk factors associated with Brucella infection in smallholders keeping large ruminants.

Res Vet Sci

February 2025

Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China; UKMC, Manchester M11 1AA, UK. Electronic address:

Brucellosis is a disease that poses a higher risk of transmission to animals and people who have close interactions with them, such as farmers. Therefore, the present study was designed to determine the seroprevalence of bovine brucellosis and associated risk factors in Kasur, Punjab, Pakistan. One hundred pooled milk samples from 100 smallholder mixed herds of cows and buffaloes with a total study population of 425 animals (277 cattle and 148 buffalos) with a range of up to seven animals in each herd were collected and tested through a milk ring test (MRT).

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Detection of infection in raw milk of livestock in Famenin, West part of Iran.

Iran J Vet Res

January 2024

Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran.

Article Synopsis
  • * A total of 738 raw milk samples were collected, with MRT revealing a 6.23% positivity rate, primarily from sheep (91.30%), though no positive results came from cattle.
  • * PCR confirmed that 78.26% of MRT-positive samples were from the brucella genus, emphasizing the need for routine raw milk assessments to identify and manage brucellosis infection effectively.
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One zoonotic infectious animal disease is brucellosis. The bacteria that cause brucellosis belong to the genus . Numerous animal and human species are affected by brucellosis, with an estimated 500,000 human cases recorded annually worldwide.

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Seroprevalence of brucellosis in communal and smallholder cattle farming in North West Province, South Africa.

Onderstepoort J Vet Res

December 2023

Onderstepoort Veterinary Research, Agricultural Research Council, Pretoria Department of Agriculture and Animal Health, College of Agriculture and Environmental Sciences, University of South Africa, Pretoria.

Brucellosis is an important bacterial zoonosis responsible for considerable economic losses in livestock and health-related burden worldwide. The objective of this study was to determine the seroprevalence of brucellosis in communal and smallholder cattle farming in four districts of the North West province of South Africa (Dr Ruth Segomotsi Mompati, Ngaka Modiri Molema, Bojanala platinum and Dr Kenneth Kaunda districts). Seven hundred and seventy blood samples from farmed animals (n = 378) and abattoir-slaughtered animals (n = 392) were collected.

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Brucellosis is a significant infection that causes abortion, decreased milk production, and sterility in livestock, which greatly affects the industry. This study aimed to determine the prevalence of Brucella in buffalo milk samples across various regions of Iran, utilizing serological, molecular, and cultural analyses. A total of 1860 buffalo milk samples were collected from industrial, semi-industrial, and traditional buffalo farms in four major buffalo breeding provinces.

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