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Transplantation of Aceh cattle ovary into the uterus of pseudopregnant local rabbits: Effect of post-transplant stress on uterine histopathology and ovarian follicle dynamics.
Vet World
March 2023
Study Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, 23111, Aceh, Indonesia.
Background And Aim: The increase in the levels of the cortisol hormone caused by the stress conditions generated by an ovary transplantation procedure can damage the uterus of the transplant recipient as well as the transplanted ovaries. This study aimed to analyze the histopathological changes that occur in the uterine horn of pseudopregnant local rabbits (recipients), as well as the ovarian follicular integrity of the donor Aceh cattle after transplantation.
Materials And Methods: After 30 days of adaptation, all rabbits were divided into three treatment groups: R1 (the group of rabbits that underwent ovarian transplantation for 3 days, n = 5), R2 (the group of rabbits that underwent ovarian transplantation for 5 days, n = 5), and R3 (the group of rabbits that underwent ovarian transplantation for 7 days, n = 5).
Gene Targeting in Rabbits: Single-Step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids.
Methods Mol Biol
February 2023
Animal Resource Laboratory, Center for Development of Advanced Medical Technology, Jichi Medical University School of Medicine, Yakushiji, Shimotsuke-shi, Tochigi, Japan.
The development of genome editing technology has allowed gene disruptions to be achieved in various animal species and has been beneficial to many mammals. Gene disruption using pluripotent stem cells is difficult to achieve in rabbits, but thanks to advances in genome editing technology, a number of gene disruptions have been conducted. This chapter describes a simple and easy method for carrying out gene disruptions in rabbits using CRISPR/Cas9 in which the gene to be disrupted is marked, the presence or absence of off-target candidates is checked, and a plasmid allowing simultaneous expression of Cas9 and sgRNA is constructed.
View Article and Find Full Text PDFAnti-Müllerian hormone concentrations in female rabbits and its relation to spay status, pseudopregnancy and ovarian follicle numbers.
Reprod Domest Anim
December 2022
Faculty of Veterinary Medicine, Clinic of Small Animal Surgery and Reproduction at the Centre for Clinical Veterinary Medicine, Ludwig Maximilian University, Munich, Germany.
Anti-Müllerian hormone (AMH), known for its role during foetal sexual differentiation, is secreted by the Sertoli cells in males and the granulosa cells in females during post-natal life. As serum AMH concentrations correlate with follicle numbers, AMH is utilized as a marker of ovarian reserve in many species. In dogs and cats, AMH is used as a diagnostic tool to determine spay or neuter status.
View Article and Find Full Text PDFCurrent Knowledge on the Multifactorial Regulation of Corpora Lutea Lifespan: The Rabbit Model.
Animals (Basel)
January 2021
Dipartimento di Medicina veterinaria, Università di Perugia, via San Costanzo 4, 06126 Perugia, Italy.
Our research group studied the biological regulatory mechanisms of the corpora lutea (CL), paying particular attention to the pseudopregnant rabbit model, which has the advantage that the relative luteal age following ovulation is induced by the gonadotrophin-releasing hormone (GnRH). CL are temporary endocrine structures that secrete progesterone, which is essential for maintaining a healthy pregnancy. It is now clear that, besides the classical regulatory mechanism exerted by prostaglandin E2 (luteotropic) and prostaglandin F2α (luteolytic), a considerable number of other effectors assist in the regulation of CL.
View Article and Find Full Text PDFImmunohistochemical localization of progesterone receptors alpha (PRA) in ovary of the pseudopregnant rabbit.
Anim Reprod
October 2019
Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Assiut University, Egypt.
Progesterone plays an important role in the reproductive function and follicular development in mammals. The aim of the present study was to examine the localization of progesterone receptor alpha (PRA) in ovary of pseudopregnant rabbit by immunohistochemical methods. Samples were collected from 14 h.
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