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New insights into the photophysical properties and interaction mechanisms of Janus green blue dye with polyanions and its applications in colorimetric visualization of loop-mediated isothermal amplification and polymerase chain reaction.
J Mater Chem B
October 2024
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, South Korea.
In this investigation, the photophysical properties and interaction mechanisms of Janus green blue (JGB) dye with polyanions were systematically studied using spectroscopic techniques. The absorption spectral analysis revealed that JGB binds cooperatively to sodium alginate, leading to dye stacking along the polymer chain. The interaction of JGB dye with DNA was characterized by the emergence of a metachromatic peak at 564 nm, indicating the formation of dye aggregates.
View Article and Find Full Text PDF[Renal cell carcinoma with colon metastases: an infrequent site for metastases].
Cir Cir
October 2009
Hospital General de Zona 1, Instituto Mexicano del Seguro Social, Campeche, Campeche.
Background: Renal cell carcinoma (RCC) represents approximately 3% of malignant tumors in adults and occurs in a M:F ratio of 1.5:1.0.
View Article and Find Full Text PDFSingle particle high resolution spectral analysis flow cytometry.
Cytometry A
August 2006
National Flow Cytometry Resource, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Background: While conventional multiparameter flow cytometers have proven highly successful, there are several types of analytical measurements that would benefit from a more comprehensive and flexible approach to spectral analysis including, but certainly not limited to spectral deconvolution of overlapping emission spectra, fluorescence resonance energy transfer measurements, metachromic dye analysis, free versus bound dye resolution, and Raman spectroscopy.
Methods: Our system utilizes a diffraction grating to disperse the collected fluorescence and side-scattered light from cells or microspheres passing through the interrogation region over a rectangular charge-coupled-device image sensor. The flow cell and collection optics are taken from a conventional flow cytometer with minimal modifications to assure modularity of the system.
Cell cycle-specific behavior of erythropoietin.
Exp Hematol
February 1996
Division of Hematology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
The murine erythropoietin-dependent erythroleukemia cell line, HCD-57, was employed to study the cell cycle-specific behavior of erythropoietin. Cell cycle duration for HCD-57 cells was approximately 12 hours and was uninfluenced by erythropoietin. Populations of HCD-57 cells synchronized in G1 by centrifugal elutriation were able to pass through one complete cell cycle in the absence of erythropoietin but, thereafter, arrested in G1 as identified by propidium iodide staining and flow cytometry.
View Article and Find Full Text PDF[Tissue basophils in vascular plexuses of the lateral ventricles of the brain in humans and various mammals].
Arkh Anat Gistol Embriol
August 1990
By means of histological and histochemical methods cell composition of vascular plexuses in the lateral ventricles of the rats, dogs and man has been investigated. Besides ependymocytes, endotheliocytes, pericytes, myocytes in the microcirculatory bed, described in literature, tissue basophils (TB) have been revealed and what is more--only in dog and man. The most variable forms and localizations of TB are observed in vascular plexuses of the lateral ventricles of the human brain.
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