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Canine monocytic ehrlichiosis (CME), induced by Ehrlichia canis, is an important infectious disease in dogs, characterized by various clinical signs and consequent immune dysfunction. This study aimed to characterize nuclear morphology, chromatin compaction, histone H3 acetylation, and DNA methylation in lymphocytes from dogs naturally infected with E. canis, compared with healthy controls.

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Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, Iran.

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Determination of DNA content as quality control in decellularized tissues: challenges and pitfalls.

Regen Biomater

October 2024

Tissue Engineering Lab, Department of Development and Regeneration, KU Leuven Campus Kulak, 8500 Kortrijk, Belgium.

Article Synopsis
  • Decellularized organs and tissues are gaining importance in regenerative medicine for organ and tissue transplants, emphasizing the need for quality control to prevent adverse reactions.
  • This study compared various methods for evaluating DNA content in skeletal muscle tissue, finding that Feulgen staining is more effective than traditional methods for detecting nuclear material.
  • Results showed that direct measurement of DNA in tissue lysates is superior to silica extraction methods, and a fluorescence-based approach yields more accurate DNA detection than UV/VIS absorbance, paving the way for improved standardization in evaluating decellularized matrices.
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Objective: The advancement of telecommunication technology and devices promptly transformed mobile phones into indispensable objects in our day-to-day lives, but their biological effects remain unclear. Therefore, this study aimed to investigate the potential histopathological changes induced by mobile phone radiation in the parotid gland and the nearby tissues.

Materials And Methods: Thirty female Rattus Norvegicus rats were divided into three groups: group 1 (exposed for 30 days), group 2 (exposed for 60 days), and control group (non-exposed).

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