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25-Hydroxycholesterol modulates synaptic vesicle endocytosis at the mouse neuromuscular junction.
Pflugers Arch
January 2025
Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2/31 Lobachevsky St, Kazan, 420111, RT, Russia.
Many synaptic vesicles undergo exocytosis in motor nerve terminals during neuromuscular communication. Endocytosis then recovers the synaptic vesicle pool and presynaptic membrane area. The kinetics of endocytosis may shape neuromuscular transmission, determining its long-term reliability.
View Article and Find Full Text PDFEffects of oil pollution on the growth and rhizosphere microbial community of Calamagrostis epigejos.
Sci Rep
January 2025
Xi'an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi'an, 710061, Shaanxi, China.
Bacteria, fungi, archaea, and viruses are reflective organisms that indicate soil health. Investigating the impact of crude oil pollution on the community structure and interactions among bacteria, fungi, archaea, and viruses in Calamagrostis epigejos soil can provide theoretical support for remediating crude oil pollution in Calamagrostis epigejos ecosystems. In this study, Calamagrostis epigejos was selected as the research subject and subjected to different levels of crude oil addition (0 kg/hm, 10 kg/hm, 40 kg/hm).
View Article and Find Full Text PDFChanges of shrimp myofibrillar proteins hydrolyzed by Virgibacillus proteases: Structural characterization, mechanism visualization, and flavor compound formation.
Food Res Int
January 2025
State Key Laboratory of Food Science and Resources, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Guangdong Engineering Research Center of High-Value Utilization and Equipment Development of Marine Biological Resources, Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong 511458, China; Jiangnan University (Shaoxing) Industrial Technology Research Institute, Shaoxing, Zhejiang 31200, China; National Engineering Research Center of Huangjiu, Zhejiang Guyuelongshan Shaoxing Wine CO., LTD, Shaoxing 646000, Zhejiang, China. Electronic address:
To explore the mechanism of Virgibacillus proteases on hydrolysis of shrimp myofibrillar protein (SMP) and formation of volatile compounds, the fermented broth of Virgibacillus halodenitrificans was purified and the protease was identified as peptidase S8. The enzyme had optimum activity at pH 7.0-8.
View Article and Find Full Text PDFUnlocking the potential of β-limit dextrin: Preparation, structure, properties, and promising applications.
Carbohydr Polym
March 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Institute of Future Food Technology, JITRI, Yixing 214200, China. Electronic address:
Starch is a widely used and economically important polymer; however, its industrial applications are limited by certain shortcomings, such as retrogradation and high digestion rate. To overcome these limitations, native starches can be hydrolyzed by β-amylase, resulting in the production of β-limit dextrin (β-LD) and maltose as a co-product. β-LD retains the original inner core structure of its parent amylopectin and contains truncated external chains that is not prone to form exterior chain helical.
View Article and Find Full Text PDFEffect of glycoside hydrolase-mediated wheat arabinoxylan hydrolysate on gut microbiota and metabolite profiles.
Carbohydr Polym
March 2025
Center of Food Colloids and Delivery for Functionality, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; China Agricultural University-Sichuan Advanced Agricultural & Industrial Institute, Sichuan, Chendu, 610046. Electronic address:
Cereal arabinoxylans (AX) are complex non-digestible polysaccharides and their molecular structural features significantly influence their degradation and metabolic behaviors within the body. This study focuses on investigating the impact of wheat AX hydrolysates produced by different glycoside hydrolases on the gut microbiota during colonic fermentation. Endo-1,4-β-xylanase (XYN) and arabinofuranosidase (ARF) were used to hydrolyze the xylan backbone and remove the arabinose side chains, respectively.
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