Influence of a low molecular weight serine proteinase inhibitor (camostate) on lipolysis of isolated adipocytes.

The effect of the epsilon-guanidino acid derivate camostate (FOY 305) on lipolysis of isolated adipocytes was evaluated to test its supposed influence on the reactions of lipid metabolism in experimental shock. Isoprenaline (10(-6) M) and theophylline (10(-3) M) significantly stimulated lipolysis in vitro. The basal submaximal (isoprenaline 10(-6)-10(-9) M) and maximal (isoprenaline 10(-6) M) catecholamine-stimulated lipolysis were not affected by camostate (10(-6) M). Furthermore, camostate (0.1-100 X 10(-6) M) did not influence half-maximal theophylline-induced lipolysis of the adipocytes. Our findings do not support the suggestion that epsilon-guanidino acid derivatives diminish in shock the increase of free fatty acids in blood via an influence on lipolysis in the peripheral adipose tissue.