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Preparation of the immobilized α-adrenoceptor column by the ultra-high affinity protein pair CL7/Im7 and its application in drug-protein interaction analysis.
J Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Northwest University Chang An Hospital, Faculty of Life Sciences and Medicine, Northwest University, Xi'an, Shaanxi 710069, China; Department of Clinical Pharmaceutics, Chang An District Hospital, Xi'an, Shaanxi 710118, China. Electronic address:
Immobilizing the target protein on a solid surface with controlled orientation, high specificity, and maintained activity is a proven strategy to enhance the stability of the protein. In this study, we employed an ultra-high affinity protein pair consisting of a mutant of colicin E7 Dnase and its corresponding inhibitor, immunity protein 7(Im7), to develop an immobilized α-adrenoceptor (α-AR) column. Briefly, we expressed α-AR fused with CL7 as a tag at its C-terminus in Escherichia coli cells.
View Article and Find Full Text PDFEncapsulation of Oil Droplets Using Film-Forming Janus Nanoparticles.
Langmuir
January 2025
School of Chemistry, Key Centre for Polymers and Colloids, The University of Sydney, Sydney, New South Wales 2006, Australia.
Polymer Janus nanoparticles with one hard cross-linked polystyrene lobe and one soft film-forming poly(methyl methacrylate--butyl acrylate) lobe were synthesized by reversible addition-fragmentation chain transfer (RAFT)-mediated emulsion polymerization. The Janus nanoparticles adsorbed to oil/water and air/water interfaces, where the soft lobes coalesced, forming films of thickness between 25 and 250 nm; droplets of silicone oil could be stably encapsulated in polymer in this way. When prepared by mechanical mixing without additives, capsules of diameter 5-500 μm could be prepared, and with additives and application of heat, capsules of diameter around 5 μm were achieved, even with highly viscous silicone oil (20,000 cSt).
View Article and Find Full Text PDFNanoscale Effects in the Room-Temperature UV-Visible Photoluminescence from Silica Particles and Its Cancer Cell Imaging.
Bioconjug Chem
January 2025
Department of Physics, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Silica nano/microparticles have generated significant interest for the past decades, emerging as a versatile material with a wide range of applications in photonic crystals, bioimaging, chemical sensors, and catalysis. This study focused on synthesizing silica nano/microparticles ranging from 20 nm to 1.2 μm using the Stöber and modified Stöber methods.
View Article and Find Full Text PDFLigand-Shell Cooperativity in a Bilayer Silica-Sandwiched Mixed-Metals Nanocatalyst Design for Absolute Selectivity Switch.
ACS Nano
January 2025
Creative Research Initiative Center for Nanospace-confined Chemical Reactions (NCCR), Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.
Unlike homogeneous metal complexes, achieving absolute control over reaction selectivity in heterogeneous catalysts remains a formidable challenge due to the unguided molecular adsorption/desorption on metal-surface sites. Conventional organic surface modifiers or ligands and rigid inorganic and metal-organic porous shells are not fully effective. Here, we introduce the concept of "ligand-porous shell cooperativity" to desirably reaction selectivity in heterogeneous catalysis.
View Article and Find Full Text PDFExploration of halogen-free sustainable superhydrophobic materials for surface protection from multi-contaminants in all weather conditions.
Mater Horiz
January 2025
New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru 560064, India.
The complex synthetic approach and utilization of toxic chemicals restrain the commercialization of numerous existing superhydrophobic materials. This article focuses on the development of a halogen-free superhydrophobic material for self-cleaning applications. HMDS-modified MCM-41 is employed as the base material.
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