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Substrates removal and growth kinetic characteristics of a heterotrophic nitrifying-aerobic denitrifying bacterium, Acinetobacter harbinensis HITLi7 at 2 °C.
Bioresour Technol
July 2018
School of Environment, Harbin Institute of Technology, Harbin, China.
In order to investigate the heterotrophic nitrification and aerobic denitrification ability of Acinetobacter harbinensis HITLi7 at 2 °C, both the growth parameters and substrates utilization characteristics were tested and appropriated kinetic models were obtained in this study. Under the initial concentration of 5 mg/L, the maximum NH-N and NO-N degradation rates were 0.076 mg NH-N/L/h and 0.
View Article and Find Full Text PDFKinetic characteristics and modelling of growth and substrate removal by Alcaligenes faecalis strain NR.
Bioprocess Biosyst Eng
April 2016
College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, 400045, People's Republic of China.
Alcaligenes faecalis strain NR has the capability of simultaneous ammonium and organic carbon removal under sole aerobic conditions. The growth and substrate removal characteristics of A. faecalis strain NR were studied and appropriate kinetic models were developed.
View Article and Find Full Text PDFTranshydrogenation reactions catalyzed by mitochondrial NADH-ubiquinone oxidoreductase (Complex I).
Biochemistry
December 2007
Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge, CB2 0XY, UK.
NADH-ubiquinone oxidoreductase (complex I) is the first enzyme of the respiratory electron transport chain in mitochondria. It conserves the energy from NADH oxidation, coupled to ubiquinone reduction, as a proton motive force across the inner membrane. Complex I catalyzes NADPH oxidation, NAD+ reduction, and hydride transfers from reduced to oxidized nicotinamide nucleotides also.
View Article and Find Full Text PDFKinetic analysis of a model for double substrate cycling: highly amplified ADP (and/or ATP) quantification.
Biophys J
June 2004
Departamento de Quimica-Fisica, Escuela Politecnica Superior de Albacete, Universidad de Castilla-La Mancha, Albacete, Spain.
Article Synopsis
- The text discusses a mathematical model describing an enzyme amplification mechanism that uses two substrate cycles, where a byproduct of the first cycle triggers the second cycle, enhancing sensitivity in measurement.
- Researchers derived time-concentration equations to analyze the species involved and demonstrated the model through a continuous assay that measures low levels of ADP and/or ATP using specific enzymes.
- The findings suggest that the method not only yields parabolic reaction progress curves but also offers equations to optimize assay costs, making it useful for low-level metabolite or enzyme activity detection in enzyme immunoassays.
Three-phase oxygen absorption and its effect on fermentation.
Adv Biochem Eng Biotechnol
February 2002
Kaposvar University, Research Institute of Chemical and Process Engineering, Veszprém, Egyetem, Hungary.
The absorption rate of oxygen in the presence of a second, dispersed, organic phase can be significantly increased due to the higher solubility and diffusivity of oxygen in the organic phase. The oxygen supply of micro-organisms, which is very often a limiting factor during fermentation, can be improved, and the critical level of oxygen in the fermentation broth can be avoided by using dispersed organic phase. This paper reviews the models of the enhanced absorption rates and their integration into mass balance equations of fermentation.
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