Various functional states of cells of sarcoma 45 in the course of its growth and regression were studied by photometry and spectral analysis using acridine orange. Fluorescent spectral analysis proved to be the most reliable procedure for quantitative evaluation of tumor cell function using the alpha-coefficient.
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The three SDEs of CLas were expressed in citrus leaves by AuNPs-PEI mediated transient expression system, and promoted the proliferation of CLas and inhibited citrus immunity. Huanglongbing (HLB) is the most severe bacterial disease of citrus caused by Candidatus Liberibacter asiaticus (CLas). CLas suppress host immune responses and promote infection by sec-dependent effectors (SDEs), thus insight into HLB pathogenesis is urgently needed to develop effective management strategies.
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The laser-induced fluorescence technique has the advantage of fast and non-destructive detection and can be used to classify types of marine microplastics. However, spectral overlap poses a challenge for qualitative and quantitative analysis by conventional fluorescence spectroscopy. In this paper, a 405 nm excitation laser source was used to irradiate 4 types of microplastic samples with different concentrations, and a total of 1600 sets of fluorescence spectral data were obtained.
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Water quality monitoring is one of the critical aspects of industrial wastewater treatment, which is important for checking the treatment effect, optimizing the treatment technology and ensuring that the water quality meets the standard. Chemical oxygen demand (COD) is a key indicator for monitoring water quality, which reflects the degree of organic matter pollution in water bodies. However, the current methods for determining COD values have drawbacks such as slow speed and complicated operation, which hardly meet the demand of online monitoring.
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