Adsorption and desorption characteristics of nucleotide based coenzymes on agarose encapsulated resins for long term cofactor supply of enzymatic reactions.

In order to find optimal long term cofactor supply for continuous enzymatic detoxification processes, different resins of varying surface, dipole moment, pore size, and chemical structure were investigated for their adsorptive capacity as well as their desorption behaviour towards various nucleotide based coenzymes. UDPGA, NADPGH, NADH, and SAM were gently shaken with agarose coated resins XAD-12, XAD-8, XAD-7, XAD-4, XAD-2, Dowex 1 X 2 (50-100; 200-400), Dowex 1 X 4 (20-50; 200-400), Dowex 2 X 8, and charcoal until all nucleotide was adsorbed or a saturation of the resins was achieved. High adsorption capacity was not always found to correlate with a steady release of cofactor in desorption experiments. Under this premise the optimal resin-cofactor combinations for long term cofactor supply were found to be XAD-12 for UDPGA, Dowex 2 X 8 for NADPH, Dowex 1 X 4 (20-50) for NADH, and XAD-7 for SAM.