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Purification and partial characterization of the allergen Ag-54 from Cladosporium herbarum. | LitMetric

AI Article Synopsis

  • * It has a molecular weight of around 20,000 daltons and is primarily a glycoprotein, consisting of 19.5% protein and 80.2% carbohydrate.
  • * The carbohydrate part includes only mannose, galactose, and glucose in a specific ratio, with no detection of amino sugars or sialic acid.

Article Abstract

One important allergen, Ag-54, was extracted from Cladosporium herbarum and purified by a combination of diafiltration, gel filtrations and isoelectric focusing. The allergen, when examined by gel electrophoresis and high-performance liquid chromatography, was found to be essentially homogeneous with a molecular weight of about 20,000 daltons. Ag-54 is of glycoprotein nature. It contained 19.5% protein, calculated from its amino acid composition and 80.2% carbohydrate, quantified by methanolysis, trimethylsilyl derivatization and gas-liquid chromatography. The carbohydrate moiety was composed only of mannose, galactose and glucose in the ratio 1.0:0.6:1.3. Frequently occurring amino sugars like glucosamine and galactosamine could not be detected. Mass spectrometry of trimethylsilyl-derivatized methylglycosides demonstrated that sialic acid was not present.

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Source
http://dx.doi.org/10.1159/000233894DOI Listing

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