The oxidative deamination of glutamate by glutamate dehydrogenase (GDH) was determined in crude homogenates of the shrimp Crangon crangon. The GDH activity of whole shrimps (1.192 +/- 0.164 UI/g wet wt and 0.032 +/- 0.004 UI mg protein) (+/- SD) is probably sufficient to account for all the ammonia excretion of this species. Starvation markedly influenced GDH activity. A 50% decrease of GDH activity was observed following 7 days of fasting but subsequently no further decrease in GDH activity was noticed during starvation up to a maximum of 17 days.
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http://dx.doi.org/10.1016/0305-0491(85)90229-9 | DOI Listing |
Microorganisms
December 2024
Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, China.
Nitrogen fertilizers in agriculture often suffer losses. Ammonia-assimilating bacteria can immobilize ammonia and reduce these losses, but they have not been used in agriculture. This study identified an ammonia-assimilating strain, sp.
View Article and Find Full Text PDFBiosensors (Basel)
December 2024
Cofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, China.
Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Technology and Business University (BTBU), Beijing 100048, China; School of Light Industry Science and Engineering, Beijing Technology and Business University (BTBU), Beijing 100048, China. Electronic address:
The chiral alcohols (S)-4-chloro-3-hydroxy-butyric acid ethyl ester ((S)-CHBE) is a critical intermediate in the synthesis of various active pharmaceutical ingredients. This study presents the first investigation of the efficient production of (S)-CHBE using organic-inorganic hybrid nanoflowers (GDH-CR@HNFs) for the co-immobilization of glucose dehydrogenase (BsGDH) and carbonyl reductase (BsCR). By optimizing immobilization conditions, we significantly enhanced the catalytic activity and immobilization efficiency of the hybrid nanoflowers.
View Article and Find Full Text PDFJ Biol Chem
January 2025
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan. Electronic address:
Many patients with diabetes use self-measurement devices for blood glucose to understand their blood glucose levels. Most of these devices utilize FAD-dependent glucose dehydrogenase (FAD-GDH) to determine blood glucose levels. For this purpose, FAD-GDHs specifically oxidizing glucose among the sugars present in blood is required.
View Article and Find Full Text PDFJ Biotechnol
January 2025
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. Electronic address:
In our previous study, the whole cells containing an aldo-keto reductase (yhdN) and glucose dehydrogenase (GDH) were constructed and applied in a stereoselective carbonyl reduction reaction to prepare (S)-NEMCA-HEPE, being a key chiral intermediate of (S)-Rivastigmine which is widely prescribed for the treatment of Alzheimer's disease. Although the conversion and enantiomeric excess (e.e.
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