A selective medium (CAP) composed of a GC agar base supplemented with 1% hemoglobin, 1% Polyvitex, and an antibiotic mixture of polymyxin B (15 U/ml), vancomycin (5 micrograms/ml), trimethoprim (2.5 micrograms/ml), and amphotericin B (2.5 micrograms/ml) was compared with another selective medium (TBBP) and two nonselective media--a blood agar and a chocolate agar--to isolate Capnocytophaga species from 725 clinical specimens. These included sputa (467 specimens), throat swabs (116 specimens), oral ulcerations (35 specimens), and periodontal pockets (107 specimens). The recovery rate of Capnocytophaga species was significantly higher with the CAP medium (96%) than with the selective TBBP medium (52.2%), the nonselective blood agar (6.2%), and the chocolate agar (4.6%). Growth of the normal flora was best inhibited on CAP medium. Colony size and yellow-brown pigment formation were maximally expressed on chocolate agar and CAP medium, but gliding motility was mostly absent. We conclude that the CAP medium is an excellent medium for the recovery of Capnocytophaga species from contaminated clinical specimens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC268413 | PMC |
| http://dx.doi.org/10.1128/jcm.22.3.375-378.1985 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clinical and Epidemiologic Review of Capnocytophaga Spp. Infections Identified at a Public Health Reference Laboratory-California, 2005-2021.
Zoonoses Public Health
January 2025
Infectious Diseases Branch, Division of Communicable Disease Control, Center for Infectious Diseases, California Department of Public Health, Sacramento, California, USA.
Introduction: Capnocytophaga is a genus of bacteria that are commensal to the oral microbiome of humans and some animals. Some Capnocytophaga species are found in the human oral cavity and rarely cause disease in people; the species found in animals are zoönotic and can be transmitted to people via saliva. This study describes the clinical and epidemiologic features of patients from whom Capnocytophaga spp.
View Article and Find Full Text PDFIn bacteria, spontaneous mRNAs degradation and ribotoxin-induced RNA damage are two main biological events that lead to the stall of protein translation. The ubiquitous trans-translation system as well as several alternative rescue factors (Arfs) are responsible for rescuing the stalled ribosomes caused by truncated mRNAs that lack the stop codons. To date, protein release factor homolog (PrfH) is the only factor known to rescue the stalled ribosome damaged by ribotoxins.
View Article and Find Full Text PDFOral Microbiota Associated with Clinical Efficacy of Ustekinumab in Crohn's Disease.
Endocr Metab Immune Disord Drug Targets
January 2025
Department of Stomatology, The Affiliated Huaian No.1 People's Hospital, Nanjing Medical University, No.1 Huanghe West Road, Huaian, 223300, Jiangsu Province, China.
Background: Crohn's Disease (CD) is a chronic inflammatory gastrointestinal disease. Ustekinumab (UST) has been utilized as a therapeutic option for CD patients. However, approximately 40-60% of patients exhibit an inadequate response to UST.
View Article and Find Full Text PDFAn overview of the microbiota of the oral cavity of humans and non-human primates with periodontal disease: Current issues and perspectives.
Arch Oral Biol
November 2024
Pathology, Science in Microbiology, Oswaldo Cruz Institute, Brazil. Electronic address:
Objective: To provide a comprehensive summary of the available evidence on the oral microbiota of humans and non-human primates about the etiology of periodontal disease.
Design: An integrative literature review was conducted on 398 clinical and observational articles published between 2010 and 2024 using searches in the MEDLINE/PubMed, Virtual Health Library, and SciELO databases. After the screening, eligibility, data extraction, and methodological quality assessment, 21 studies were selected.
Capnocytophaga canimorsus in Iliac Artery Mycotic Aneurysm: The Role of Molecular Diagnostics.
Am J Case Rep
January 2025
Vascular and Endovascular Surgery, Department of Cardiovascular Surgery, Mayo Clinic Health System, Eau Claire, WI, USA.
BACKGROUND The bacterial organism Capnocytophaga canimorsus is an oral commensal of cats and dogs and can cause life-threatening infections like mycotic aneurysm, meningitis, and sepsis. Mycotic aneurysms occur when microbial infections cause arterial wall degeneration. Difficulty in diagnosing Capnocytophaga canimorsus infection can occur due to the bacteria's fastidious nature and laboratory testing limitations, contributing to the infection's high morbidity and mortality.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!