A screening method was established to develop chemically defined, serum-free culture media for human adherent cell lines. It indicates within 2-6 months whether a certain cell line is suited for routine cultivation under serum-free conditions, and which medium supplements and surface coatings of the culture vessels the cells need for minimal demand. This method was tested with the human cell lines Bowes melanoma and prostate carcinoma 3 (PC 3).
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