The quantitative regional distribution of neuromedin B-like immunoreactivity in normal postmortem human spinal cord was studied by a specific radioimmunoassay. Neuromedin B-like immunoreactivity was found in highest concentration in the dorsal part of the sacral cord. Chromatographic analyses by gel permeation and reverse-phase high-pressure liquid chromatography revealed two major peaks of neuromedin B-like immunoreactivity and the prevalent molecular form, approx. 90% of the total immunoreactivity, was chromatographically identical to synthetic porcine neuromedin B.
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The presence, characterisation and synthesis of neuromedin B in the human pituitary gland.
Neuroendocrinology
November 1992
Department of Medicine, Royal Postgraduate Medical School, London, UK.
Neuromedin B is a 10-amino-acid mammalian peptide of the bombesin family. We have used a specific radioimmunoassay and Northern blot hybridisation to investigate the possible synthesis of neuromedin-B-like immunoreactivity in the human pituitary gland. The concentration of immunoreactive neuromedin B in whole human pituitary was 15.
View Article and Find Full Text PDFNeuromedin B-like immunoreactivity in the brain of the green frog (Rana esculenta L.).
Eur J Basic Appl Histochem
April 1992
Dipartimento di Biologia Animale, Università di Torino.
Three rabbit polyclonal antisera, originally developed against neuromedin B and highly selective against ranatensin subfamily molecules, were used to study the distribution of neuromedin B-like immunoreactivity in the brain of Rana esculenta. Immunopositive cell bodies were observed in several brain regions, including medial and lateral septal nuclei, nucleus of the diagonal band of Broca, medial amygdala, ventral striatum, ventromedial and posterior thalamic nuclei, nucleus of the periventricular organ, posterior tuberculum, dorsal, lateral and ventral hypothalamic nuclei, optic tectum, mesencephalic tegmentum and central rhomboencephalic gray. A dense network of immunopositive fibers was also distributed in defined regions of the frog brain, i.
View Article and Find Full Text PDFdes-Met carboxyl-terminally modified analogues of bombesin function as potent bombesin receptor antagonists, partial agonists, or agonists.
J Biol Chem
September 1990
Digestive Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
In the present study we examined the effect of carboxyl-terminal modifications of des-Met14-bombesin (Bn) on Bn receptor affinity in murine 3T3 cells, rat and guinea pig pancreatic acini, and the ability to initiate biologic responses by synthesizing 18 des-Met14-Bn(6-13) analogues. With guinea pig acini and 3T3 cells, affinity was affected by the chain length of the alkyl moiety (R) added to [D-Phe6]Bn(6-13)NH2R with relative potencies: propyl greater than ethyl greater than butyl = hexyl greater than heptyl greater than free amide, whereas in rat acini affinity was not increased by the chain length. In each cell system the affinity of the alkylamide was not increased by insertion of a phenyl group in the alkyl side chain, by making the analogue more neuromedin B-like or by addition of a reduced peptide bond.
View Article and Find Full Text PDFNeuromedin B-like peptides were characterized in the rat brain. A rabbit antisera was utilized which recognized neuromedin B but not bombesin or GRP. Using gel filtration and HPLC techniques, a major and minor peak of immunoreactivity was present in rat brain extracts.
View Article and Find Full Text PDFThe presence of immunoreactive (ir)-bombesin in bovine adrenal medulla, isolated adrenal chromaffin cells and subcellular fractions of the adrenal medulla was demonstrated using a specific antibody to the synthetic peptide. High levels of ir-bombesin were detected in acid (HCl) extracts of the adrenal tissue (27 pmol/g) and isolated cells (0.35 pmol per 10(6) cells).
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