The immunoprecipitation reaction for the determination of microamounts of alpha1-antitrypsin was conducted in the capillaries filled with a microvolume of a 1% agarose gel (the gel length was 7--8 mm, and the diameter--0.6 mm). Microamounts of the antigen and antiserum were applied to the surface of the opposite ends of the gel. Precision control of these manipulations was carried out by means of an ocular micrometer of a binocular lens. The amount of the formed immunoprecipitate in gel was calculated by the summated optic density after staining with amido black recorded by the method of double-wave microspectrophotometry. Sensitivity of the method was 0.3--1.0 ng of alpha1-antitrypsin. The linear plot between the amount of alpha1-antitrypsin and the amount of the precipitate formed was found within the range of 1--25 ng of protein. The error was about 10%.
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Forensic Sci Int
May 2023
Department of Chemistry, University of Central Florida, Orlando, FL, USA; National Center for Forensic Science, University of Central Florida, Orlando, FL, USA. Electronic address:
Within the past two decades, there have been many studies for quantitative analysis on human hair samples. Microscopical and chemical analysis techniques have been used to analyze various aspects of hair regarding biological, chemical, anthropological, cosmetic, and forensic applications. Studies have attempted to develop quantification methods to increase the evidentiary value of hair in forensic casework.
View Article and Find Full Text PDFJ Biomech
March 2021
Univ Lyon, Université Claude Bernard Lyon 1, INSERM, LYOS UMR 1033, 69008 Lyon, France. Electronic address:
A finite element analysis based on Micro-Quantitative Computed Tomography (µQCT) is a method with high potential to improve fracture risk prediction. However, the segmentation process and model generation are generally not automatized in their entirety. Even with a rigorous protocol, the operator might add uncertainties during the creation of the model.
View Article and Find Full Text PDFJ Bone Miner Res
June 2014
Department of Medicine, Hematology Oncology, Indiana University, Indianapolis, IN.
Measles virus nucleocapsid protein (MVNP) expression in osteoclasts (OCLs) and mutation of the SQSTM1 (p62) gene contribute to the increased OCL activity in Paget's disease (PD). OCLs expressing MVNP display many of the features of PD OCLs. Interleukin-6 (IL-6) production is essential for the pagetic phenotype, because transgenic mice with MVNP targeted to OCLs develop pagetic OCLs and lesions, but this phenotype is absent when MVNP mice are bred to IL-6(-/-) mice.
View Article and Find Full Text PDFOsteoarthritis Cartilage
March 2009
Children's Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.
Objective: Characterize the effects of alendronate (ALN) on the repair process of the osteonecrotic femoral head as well as the development of secondary osteoarthritis in the ipsilateral hip in an established experimental model of osteonecrosis.
Methods: Osteonecrosis of the femoral head was induced surgically in 60 adult, male New Zealand white rabbits. Animals were randomized in two placebo- (saline) and two treatment-groups (ALN 150 microg/kg/day S.
Anal Chim Acta
January 2007
Faculty of Pharmacy, Department of Physical Chemistry, University of Belgrade, Vojvode Stepe 450, YU-11000 Belgrade, Serbia and Montenegro.
A novel kinetic method for micro-quantitative determinations of morphine (MH) is proposed and validated. The method is based on the potentiometric monitoring of the concentration perturbations of the oscillatory reaction system being in a stable non-equilibrium stationary state close to the bifurcation point between stable and oscillatory state. The response of the Bray-Liebhafsky (BL) oscillatory reaction as a matrix system, to the perturbations by different concentrations of morphine, is followed by a Pt-electrode.
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