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Cryptococcosis predominantly affects immunocompromised individuals, particularly those with advanced HIV disease, with meningitis being the most severe form and linked to high mortality. Diagnosis typically relies on rapid Cryptococcus antigen (CrAg) testing, and antigen titer quantification helps in early detection and assessing disease severity. However, conventional titer methods are often more expensive than qualitative antigen detection.

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Fresh-cut taro, renowned for its high nutritional value and convenience, is prone to rapid browning post-cutting, which hinders its storage life. This study focused on the effects of L-ascorbic acid (AA) combined with ultrasound (US) treatment (AS) on the storage quality and transcriptome analysis of fresh-cut slices of Yongding June Red Taro. Compared to the control (CK) group, AS treatment effectively reduced the weight loss rate of taro slices, maintained higher hardness, delayed the increase of browning, and inhibited the accumulation of O and HO.

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Aims: () is a rare medicinal mushroom that is rich in polysaccharides, triterpenes, flavonoids, and other bioactive compounds. It has good potential development value.

Methods And Results: We performed single factor experiments and Box-Behnken response surface methodology to optimize the liquid fermentation medium formulation for with mycelial biomass as the indicator.

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Improvement of a MALDI-TOF database for the reliable identification of (formally ) and related species.

Microbiol Spectr

January 2025

School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding, Hebei, China.

Article Synopsis
  • The study focuses on developing a MALDI-TOF MS database for the accurate identification of various species from a specific microorganism complex, overcoming previous misidentifications.
  • It involved analyzing 71 isolates, creating a new database with 37 strains using a formic acid extraction method, and validating it with an additional 34 strains.
  • The database successfully achieved 100% identification accuracy, showing that MALDI-TOF MS is a reliable tool for diagnosing infectious agents in clinical settings.
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Treatment of onychomycosis includes topical and systemic agents. However, prolonged use of oral treatment could cause adverse effects and topical antifungal agents have limited penetration. To compare the clinical efficacy and the safety of fractional CO laser combined with topical tioconazole nail solution versus Q-switched 1064 Nd: YAG laser in the treatment of fingernail onychomycosis.

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