A simple method of detecting gluten antibodies in serum is described. Cryostat sections of wheat grains proved to be an excellent substrate in the immunofluorescence technique. Rabbit antisera to gliadin and alpha-gliadin, and high percentage of sera from patients with gluten-induced enteropathy had antibodies that reacted with an internal structure of wheat grains. These antibodies could be absorbed with gliadin and with alpha-gliadin.
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http://dx.doi.org/10.1016/s0022-1759(97)90025-9 | DOI Listing |
Sci Rep
January 2025
ICAR-Indian Institute of Millets Research, Rajendranagar, Hyderabad, 500 030, Telangana, India.
The gradual increase in the consumption of mineral nitrogen is leading to heightened levels of harmful air pollutants, particularly NO emissions from the agriculture sector. A potential solution to address the issues arising from the excessive use of urea in wheat is the substitution of conventional urea with nano urea. This study aimed to quantify the effects of nano urea, both independently and in conjunction with prilled urea, under various agroclimatic and sowing conditions in India.
View Article and Find Full Text PDFTalanta
January 2025
Engineering Research Center of Grain Storage and Security of Ministry of Education, Henan Provincial Engineering Technology Research Center on Grain Post Harvest, School of Food and Strategic Reserves, Henan University of Technology, Lianhua Road 100, Zhengzhou High-Tech Development Zone, Zhengzhou, 450001, Henan, China. Electronic address:
Aflatoxin B1 (AFB1) has strong carcinogenicity, mutagenicity, and teratogenicity even at low concentrations, presenting a major risk to food safety and human health, hence, it is crucial to develop a sensitive detection technique for AFB1. Consequently, cadmium telluride (CdTe) quantum dots conjugated with AFB1 aptamers serve as fluorescent signal probes, whereas FeO@UiO-66-NH nanocomplexes are employed as magnetic carriers and fluorescence quenchers. FeO@UiO-66-NH reduces background signal interference, thereby enhancing detection sensitivity and Förster Resonance Energy Transfer (FRET) efficiency.
View Article and Find Full Text PDFPoult Sci
January 2025
Department of Animal Science, University of Zabol, Zabol 98661-5538, Iran. Electronic address:
Calcium (Ca) is necessary for bone health and metabolic functions in poultry, however, the extent to which it can be utilized varies among feed ingredients. The goal of this study was to determine the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of calcium in wheat and soybean meal (SBM) in young quail chicks using a direct method. Three dietary treatments were used in the experiment: a calcium-free basal diet to determine endogenous calcium losses, and two diets with either wheat or SBM as the primary calcium sources.
View Article and Find Full Text PDFPlant Physiol Biochem
January 2025
Agricultural College, Anhui Agricultural University, 230036, Hefei, China; Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry (CIC-MCP), 210095, Nanjing, China. Electronic address:
Nitric oxide (NO) positively contributes to maintaining a high photosynthetic rate in waterlogged-wheat plants by maintaining high stomatal conductance (g), mesophyll conductance (g), and electron transport rates in PSII (J). However, the molecular mechanisms underlying the synergistic regulation of photosynthetic characteristics during wheat waterlogging remain unclear. Pot experiments were conducted with two cultivars: Yangmai15 (YM15: high waterlogging-tolerance capacity) and Yangmai24 (YM24: conventional waterlogging-tolerance capacity).
View Article and Find Full Text PDFPlant Dis
January 2025
Hebei Academy of Agricultural and Forestry Sciences, Plant Protection Institute, 437 Dongguan Street, Baoding, Hebei, China, 071000.
Strawberry () is an important economic crop in Hebei, China. In May 2023, root rot was observed in strawberry plantations (cultivar 'Benihoppe') in Shijiazhuang (37°57'23″N, 115°16'34″E), Hebei, China. The incidence of the disease reached up to 30% in the field.
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