The degradation of extracellular matrix proteins such as collagen and elastin with aging leads to skin sagging. Polycaprolactone (PCL) microspheres are used as facial fillers because of their ability to provide volume, biodegradability, and collagen-stimulating properties. The direct biological effects of PCL microspheres on fibroblasts, particularly in stimulating sustained collagen production, require further investigation. We detected the safety and effect of PCL microspheres on human fibroblasts and investigated new collagen synthesis and the thickness of C57BL/6 mouse skin. Through an RNA-seq analysis of differentially expressed genes, we identified a key regulator of collagen production in PCL-stimulated fibroblasts. Our research revealed that PCL microspheres are safe for human fibroblasts, promoting their proliferation and increasing new collagen synthesis and skin thickness. We identified sterile alpha motif domain containing 11 (SAMD11) as a key regulator of collagen production in PCLstimulated fibroblasts through an RNA-seq analysis. By increasing SAMD11 expression, PCL microspheres increase collagen synthesis and rejuvenate skin through the upregulation of procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (PLOD1). This study elucidates the mechanism by which SAMD11 regulates the effects of PCL microspheres as collagen stimulants for skin rejuvenation, providing a foundation for the future development and refinement of similar materials.
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Increasing collagen synthesis in fibroblasts: The roles of PCL microspheres and the SAMD11-PLOD1 axis in skin rejuvenation.
Biochim Biophys Acta Mol Cell Res
March 2025
The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China; Department of Plastic and Reconstructive Surgery, Guangdong Second Provincial General Hospital, Guangzhou, China. Electronic address:
The degradation of extracellular matrix proteins such as collagen and elastin with aging leads to skin sagging. Polycaprolactone (PCL) microspheres are used as facial fillers because of their ability to provide volume, biodegradability, and collagen-stimulating properties. The direct biological effects of PCL microspheres on fibroblasts, particularly in stimulating sustained collagen production, require further investigation.
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Electrosprayed core-shell microspheres co-deliver fibronectin and resveratrol for combined treatment of acute lung injury.
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State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620 China; CQM-Centro de Química da Madeira, Universidade da Madeira, Campus Universitário da Penteada 9020-105 Funchal, Portugal. Electronic address:
The facile development of advanced formulations capable of scavenging excess reactive oxygen species (ROS) and sustainably inhibiting inflammatory cytokine secretion is imminent for effective treatment of acute lung injury (ALI), but still remains a great challenge. This study presents an innovative core-shell carrier system via electrospray technology, characterized by a shell of poly(lactic acid-co-glycolic acid) (PLGA) and a core consisting of polycaprolactone-polyethylene glycol (PCL-PEG) micelles encapsulating resveratrol (Res), and surface modified with fibronectin (FN). The created drug-loaded core-shell microspheres (for short, RPG@FN) with a size of 1.
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