Fructus Mori polysaccharides modulate the axial distribution of gut microbiota and fecal metabolites to improve symptoms of hyperglycemia in type 2 diabetic mice.

To understand the gut microbiota composition in different intestinal segments is essential to clarify the structure-hypoglycemic relationship of Fructus Mori Polysaccharides (MFP). In this study, the spatial distribution of gut microbiota and fecal metabolites affected by MFP in type 2 diabetes mice was investigated using the 16S rRNA high-throughput sequencing technology and gas chromatography. The results showed that MFP could control body weight and reduce the blood glucose level. After MFP intervention, the abundance of Lactobacillus and Bifidobacterium increased by 3.43 and 33.47 times in the ascending colon mucus layer, and Prevotella increased by 102.25 times in the transverse colon content, while Helicobacter and Treponspirillum was reduced by 99.28 % and 79.38 % in the ascending colon mucus layer, and Ruminococcus reduced by 68.77 % in the cecum contents. Moreover, the MFP increased the contents of short-chain fatty acids (SCFAs) with significantly difference along the longitudinal axis of intestine. The results suggested that the MFP relieve the hyperglycemia of diabetic may through modulating the microorganism in ascending colon mucus layer, transverse colon content and cecum.