Objective: The purpose of the study was to investigate the cytotoxicity, biocompatibility, and osteogenic effect of EndoSequence BCSealer HiFlow (BCH) and Bio-C Sealer (BCS) in osteosarcoma cells SAOS-2 compared with AH Plus Jet.
Materials And Methods: For cytotoxicity analysis, the [3-(4,5-dimethyl-thiazole)-2,5-diphenyltetrazolium bromide-MTT; Sigma/Aldrich] method was used after 24, 48, and 72 hours. For cellular bioactivity, alkaline phosphatase enzyme (ALP) was evaluated after 7 and 14 days.
Statistical Analysis: MTT assay was assessed using analysis of variance (ANOVA) and Tukey's test and over time using ANOVA and Sidak's test. For ALP analysis, the one-way ANOVA and Student's paired t-tests were used.
Results: BCS was like AH Plus Jet. After 72 hours, BCH and BCS did not differ from AH Plus Jet and showed similar behavior in terms of viability. ALP showed a difference only after 14 days. BCH had the lowest bioactivity value. AH Plus Jet and BCS were like each other.
Conclusion: AHP was more viable for SAOS-2, and the biocompatibility of Calcium silicate-based sealers was acceptable. The present finding assessed the biocompatibility of Calcium silicate bioceramic sealers, such as BCS, EndoSequence BCH, and AH Plus Jet, is acceptable and they have osteogenic effect and bioactivity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1055/s-0044-1789602 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the potential of incorporating ZIF-67 into electrospun poly (vinyl alcohol)/chitosan nanofibrous mats for wound healing.
Int J Biol Macromol
March 2025
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
The current research emphasis is on the development of wound dressings that can inhibit bacterial infections and facilitate the treatment of complex wound healing processes. In this study, nanofibrous mats of polyvinyl alcohol/chitosan/ZIF-67(PVA/Cs/ZIF-67) were prepared using an electrospinning technique, to investigate their antibacterial and regenerative properties in a rat model of full-thickness skin wounds. ZIF-67 nanoparticles, with an average size of approximately 373.
View Article and Find Full Text PDFEvaluation of the Cytotoxicity of a Newly Developed Obturating Material for Pulpectomy in Primary Teeth Using Embryonic Toxicology, Brine Shrimp Lethality, and MTT Assay: An In Vitro Study.
Eur J Dent
March 2025
Division of Pediatric Dentistry, Department of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Objective: The search for an ideal obturating material has taken precedence due to the revolution of the materials used in pediatric endodontics. With zinc oxide, calcium hydroxide, and metronidazole as its core constituents, an unique obturating material was developed. To aid in the healing process, the material should be biocompatible and most importantly it should not have any negative consequences.
View Article and Find Full Text PDFIn vitro Evaluation of Cytotoxicity, Biocompatibility, and Osteogenic Effect of Two Calcium Silicate Sealers Compared with AH Plus Jet.
Eur J Dent
March 2025
Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Objective: The purpose of the study was to investigate the cytotoxicity, biocompatibility, and osteogenic effect of EndoSequence BCSealer HiFlow (BCH) and Bio-C Sealer (BCS) in osteosarcoma cells SAOS-2 compared with AH Plus Jet.
Materials And Methods: For cytotoxicity analysis, the [3-(4,5-dimethyl-thiazole)-2,5-diphenyltetrazolium bromide-MTT; Sigma/Aldrich] method was used after 24, 48, and 72 hours. For cellular bioactivity, alkaline phosphatase enzyme (ALP) was evaluated after 7 and 14 days.
Potential application of acid-form sophorolipids in cell cryopreservation.
Cryobiology
March 2025
Saraya Research Institute, Saraya Co., Ltd, Kashiwara, Osaka, 582-0028, Japan.
In cryopreservation, which is an important process for the long-term storage and transport of cells, cryopreservation solutions containing cryoprotectants are usually used to protect cells from damage. However, most cryoprotectants have non-negligible cytotoxicity and side effects that greatly limit their applications in clinical use. This has led to an increased need for more biocompatible cryoprotectants, and interest in bioinspired cryoprotectants is increasing.
View Article and Find Full Text PDFBiological evaluation of a new highly sensitive and selective fluorescent probe for hypochlorous acid and its imaging application in cell and zebrafish.
Bioorg Chem
March 2025
Biology Institute, Bioengineering Department, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China. Electronic address:
Hypochlorous acid is one of the most widely distributed reactive oxygen species in vivo. It is usually used as a signal molecule to participate in various life activities such as immunity and metabolism, and plays a notable role in maintaining homeostasis. When hypochlorous acid level is abnormal in the body, it will lead to a variety of diseases, such as Parkinson's disease, Alzheimer's disease, atherosclerosis and cancer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!