sp. nov., isolated from L. rhizosphere in Mexico and Sauvalle root nodule in Taiwan.

Int J Syst Evol Microbiol

Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Laboratorio de Biotecnología Microbiana. Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, Ciudad de México, Alcaldía Miguel Hidalgo. C.P. 11340, Mexico.

Published: March 2025

Several and strains from the north of Mexico were isolated during the description of strains. , and were later described as novel species. Next, the Genomic Encyclopedia of Bacteria and Archaea project retrieved the genome sequences of several strains from the above species. Standard comparative genomic analysis showed that MLR2-44 was taxonomically misclassified. This strain, isolated from the rhizosphere, was analysed by average nucleotide identity (ANI), revealing that was the closest species (95.6%). However, the digital DNA-DNA hybridization (dDDH) resulted in values lower than 68% for any type strain of species. Moreover, it was also found that LMG 19430, isolated from root nodules, was closer to strain MLR2-44 (99.9% ANI and 99.8% dDDH), instead of being associated with the type strain of LMG 19424 (94.4% ANI and 54.6% dDDH), thus making strains MLR2-44 and LMG 19430 a single genomic species. Accordingly, the polyphasic analysis showed that these strains represent a new species; therefore, we propose that strains MLR2-44 (=CDBB B-2066=TSD-312) and LMG 19430 encompass a novel species with the name of sp. nov.

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http://dx.doi.org/10.1099/ijsem.0.006709DOI Listing

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