An innovative light scattering immunoassay was developed using an AuNPs etching strategy. Three types of anisotropic gold nanoparticles, including gold nanocubes, nanorods, and nanoflowers with distinct morphologies, were utilized to investigate how these morphological differences affect the sensitivity of light scattering signal transduction. Based on theoretical insights into light scattering and electromagnetic fields, gold nanocubes were identified as the optimal probes for enhancing light scattering signal transduction and were employed to construct an immunoassay for detecting staphylococcal enterotoxin A (SEA). The developed immunoassay achieved ultrahigh sensitivity for SEA detection in milk samples, with a detection limit of 10.39 pg mL, which is 190 times lower than that of conventional ELISA. The proposed immunoassay was validated across ten food samples, demonstrating high accuracy and robustness. Given these promising results, we believe this method has significant potential for screening trace levels of SEA in food products.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.foodchem.2025.143713 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural Features of the Thymol-Carvacrol Equimolar Mixture: X-Ray Scattering and Molecular Dynamics.
J Phys Chem B
March 2025
Department of Chemistry, University of Rome Sapienza, Rome 00185, Italy.
We present a structural characterization of a low-transition-temperature mixture (LTTM), consisting of thymol and carvacrol, at an equimolar ratio. Carvacrol and thymol are natural regioisomers of terpenes. When combined at an equimolar ratio, they form a liquid mixture at room temperature, with supercooling capability and glass transition at ca.
View Article and Find Full Text PDFThe fusion kinetics of block copolymer micelles in dilute solutions have been investigated. As a model system, 1,2-polybutadiene--poly(ethylene oxide) micelles in the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate have been studied. The ionic liquid is a selective solvent for poly(ethylene oxide), promoting the self-assembly of the block copolymer into spherical micelles.
View Article and Find Full Text PDFCharging and Aggregation of Nano-Clay Na-Montmorillonite in the Presence of Ciprofloxacin.
Nanomaterials (Basel)
March 2025
Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, Japan.
The transport and fate of antibiotics are significantly influenced by co-existing colloidal and nanosized substances, such as clay particles. Montmorillonite, a common clay mineral with a thin nano-sheet-like structure, enhances antibiotic (e.g.
View Article and Find Full Text PDFNatural-Synthetic Hybrid Nanostructures Formed Through the Interaction of Chitosan with Carboxylate-Ended PNIPAM: Structure and Curcumin Encapsulation.
Nanomaterials (Basel)
February 2025
Petru Poni Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487 Iasi, Romania.
Chitosan is widely used in drug delivery applications, due to its biocompatibility, bio-degradability, and low toxicity. Nevertheless, its properties can be enhanced through the physical or chemical modification of its amino and hydroxyl groups. This work explores the electrostatic complexation of two chitosan samples of differing lengths with two poly(-isopropylacrylamide) (PNIPAM) homopolymers of different molecular weight carrying a chargeable carboxyl end group.
View Article and Find Full Text PDFAdvancing rapid diagnostics for bloodstream infections: a perspective on scattered light integrated collection technology.
J Med Microbiol
March 2025
Department of Microbiology, Symbiosis Medical College for Women (SMCW) & Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University), Lavale,, Pune, Maharashtra, India.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!