Whole-cell patch clamp allows the evaluation of neuronal excitability and characterization of synaptic transmission. With this technique, it is possible to characterize the neuron maturation level and its integration into the hippocampal circuit. This facilitates the identification of the different stages of neural progenitor cells in the adult brain and their contribution to hippocampal function.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-4386-0_18 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Objective: To analyze the effects of multiplane reconstruction (MPR) technology with multi-slice spiral CT (MSCT) in the etiological diagnosis of acute intestinal obstruction (AIO). Obtaining clear images is of great help in determining the type and etiology of AIO, and doctors can quickly develop treatment plans to improve prognosis and efficacy.
Methods: The clinical data of patients with suspected AIO admitted to our hospital from May 2020 to May 2022 were retrospectively selected as the observation objects.
Fluorescent PyrAte-()-citalopram conjugates enable imaging of the serotonin transporter in living tissue.
Chem Sci
March 2025
Centre of Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna Währinger Straβe 13A 1090 Vienna Austria
Fluorescent labeling techniques have enabled the visualization of various biomolecules, cellular structures, and their associated physiological processes. At the same time, there remains a demand for developing novel fluorescent compounds possessing unique chemical properties for biological imaging. A recently developed class of fluorophores, termed , displays optimal brightness and large Stokes shifts that are ideal for fluorescence microscopy.
View Article and Find Full Text PDFDual action of sphingosine 1-phosphate pathway in in vitro models of global cerebral ischemia.
Neurobiol Dis
March 2025
Dept. of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Florence, Italy. Electronic address:
It is well accepted that sphingolipids play an important role in the pathological process of cerebral ischemia. In the present study we have investigated the involvement of sphingosine 1-phosphate (S1P) pathway in two different in vitro models of global ischemia. In organotypic hippocampal slices exposed to oxygen and glucose deprivation (OGD) we evaluated the mRNA expression of S1P metabolic enzymes and receptors (S1P) by Real Time-PCR.
View Article and Find Full Text PDFAcute Slice Patch-Clamp Recordings to Characterize Neural Populations in the Hippocampal Subgranular Zone.
Methods Mol Biol
March 2025
Departamento de Fisiología, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Whole-cell patch clamp allows the evaluation of neuronal excitability and characterization of synaptic transmission. With this technique, it is possible to characterize the neuron maturation level and its integration into the hippocampal circuit. This facilitates the identification of the different stages of neural progenitor cells in the adult brain and their contribution to hippocampal function.
View Article and Find Full Text PDFPatch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes.
Methods Mol Biol
March 2025
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, Madrid, Spain.
Patch-clamp recordings are a fundamental technique in neuroscience to study the electrical activity of individual cells. When applied to isolated neural cells, acute tissue slices, or brain rosettes, this method provides valuable insights into neuronal function and network activity in different experimental models. Here, we describe a detailed protocol to perform patch-clamp recording in those cell preparations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!