Patch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes.

Methods Mol Biol

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, Madrid, Spain.

Published: March 2025

Patch-clamp recordings are a fundamental technique in neuroscience to study the electrical activity of individual cells. When applied to isolated neural cells, acute tissue slices, or brain rosettes, this method provides valuable insights into neuronal function and network activity in different experimental models. Here, we describe a detailed protocol to perform patch-clamp recording in those cell preparations.

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http://dx.doi.org/10.1007/978-1-0716-4386-0_17DOI Listing

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