Patch-clamp recordings are a fundamental technique in neuroscience to study the electrical activity of individual cells. When applied to isolated neural cells, acute tissue slices, or brain rosettes, this method provides valuable insights into neuronal function and network activity in different experimental models. Here, we describe a detailed protocol to perform patch-clamp recording in those cell preparations.
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http://dx.doi.org/10.1007/978-1-0716-4386-0_17 | DOI Listing |
Acta Physiol (Oxf)
April 2025
Department of Physiology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
Aim: Mitochondria play key roles in neuronal activity, particularly in modulating agouti-related protein (AgRP) and proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARC), which regulates food intake. FAM163A, a newly identified protein, is suggested to be part of the mitochondrial proteome, though its functions remain largely unknown. This study aimed to investigate the effects of Fam163a knockdown and mitochondrial dysfunction on food intake, AgRP neuron activity, and mitochondrial function in the hypothalamus.
View Article and Find Full Text PDFJ Cell Mol Med
March 2025
Fu Jen Catholic University, School of Medicine, New Taipei City, Taiwan.
Phosphodiesterase inhibitors regulate intracellular Ca of cardiomyocytes through enhancing second messenger signalling. This study aimed to investigate whether TP-10, a selective phosphodiesterase10A inhibitor, modulates Ca cycling, attenuating arrhythmogenesis in the right ventricular outflow tract (RVOT). Right ventricular tissues from New Zealand white rabbits were harvested, and electromechanical analyses of ventricular tissues were conducted.
View Article and Find Full Text PDFMethods Mol Biol
March 2025
Departamento de Fisiología, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Whole-cell patch clamp allows the evaluation of neuronal excitability and characterization of synaptic transmission. With this technique, it is possible to characterize the neuron maturation level and its integration into the hippocampal circuit. This facilitates the identification of the different stages of neural progenitor cells in the adult brain and their contribution to hippocampal function.
View Article and Find Full Text PDFMethods Mol Biol
March 2025
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, Madrid, Spain.
Patch-clamp recordings are a fundamental technique in neuroscience to study the electrical activity of individual cells. When applied to isolated neural cells, acute tissue slices, or brain rosettes, this method provides valuable insights into neuronal function and network activity in different experimental models. Here, we describe a detailed protocol to perform patch-clamp recording in those cell preparations.
View Article and Find Full Text PDFMater Today Bio
April 2025
Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino-IRCCS, Italy.
Induced pluripotent stem cells (iPSCs), carrying the patient's genetic background, open the path to advanced modeling. The feasibility of recapitulating complex pathophysiological scenarios depends on iPSC's ability to differentiate into the plurality of specific organ resident cells, on their maturation and networking. To this end, a strong interest has arisen in organoids, 3D structures, obtained by exploiting iPSC natural capability to self-assemble and rebuild organ parts.
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