This research introduces innovative tools for studying embryonic neurogenesis in the gecko Paroedura picta. Traditional research methods have been adapted for the gecko's unique biology, including variations of birthdating techniques and the implementation of EdU for tracking neuron generation. We also employ hybridization chain reaction (HCR) to detect specific mRNAs without the need for species-specific antibodies, providing a powerful and flexible tool for studying gene expression patterns in the gecko embryonic brain. However, HCR can also be combined with immunohistochemistry to visualize both RNA and protein distribution. Our work proves the importance of this innovative toolset to understand the evolutionary aspects of nervous system development and the gecko as a valuable model for understanding the ancestral stem amniote.
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