Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine.In this chapter, we describe a procedure of isolation and culture of ovarian cortical cells that consistently allows the spontaneous generation of a large number of neurospheres (NS), and the methods to demonstrate that their integrating NSC/NPC self-renew and are able to differentiate into neurons and glia, during the NS assay.
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Cell Population Analyses of Neurospheres Generated In Vitro from Ovarian Cortical Cells in Mammals: Characterization by Immunohistochemistry and Flow Cytometry.
Methods Mol Biol
March 2025
Department of Physiology, School of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.
Alternative experimental models based upon isolation and culture of extra-neural NSC/NPC or adult stem cells that can be induced in vitro to generate these cells and NS rely on development of procedures that can demonstrate the identity of cells as NSC/NPC and their compromise with the neural lineage during culture. Gene expression analyses by RT-PCRQ, immunohistochemical localization of characteristic NSC/NPC antigens, analyses of cell populations by flow cytometry, or ultrastructural analyses of in vitro generated NS are the most frequent and reliable methods to demonstrate the molecular and structural hallmarks of NSC/NPCs and NS. Among all these techniques, those aimed to immunolocalize specific NSC/NPC antigens on a cell basis and in a time-dependent fashion throughout culture require skillful NS processing before setting up the technique.
View Article and Find Full Text PDFNeurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay.
Methods Mol Biol
March 2025
Department of Physiology, School of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.
Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine.In this chapter, we describe a procedure of isolation and culture of ovarian cortical cells that consistently allows the spontaneous generation of a large number of neurospheres (NS), and the methods to demonstrate that their integrating NSC/NPC self-renew and are able to differentiate into neurons and glia, during the NS assay.
View Article and Find Full Text PDFKey Events in the Process of Sex Determination and Differentiation in Early Chicken Embryos.
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Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China, Yangzhou, China.
Clarifying the intricate mechanisms underlying sex determination and differentiation regulation in chickens holds significant potential for advancing chicken sex control technologies. This endeavor not only augments fundamental research in developmental biology but also bolsters the economic viability of the poultry industry. Our investigation delved into the sex determination and differen-tiation molecular underpinnings of these processes during the early stages of chicken embryonic development E0 d blastocysts, E3.
View Article and Find Full Text PDFDirect communication between the hippocampus and cerebellum has been shown via coactivation and synchronized neuronal oscillations in animal models. Further, this novel cerebello-hippocampal circuit may be impacted by sex steroid hormones. The cerebellum and hippocampus are dense with estradiol and progesterone receptors relative to other brain regions.
View Article and Find Full Text PDFAnti-GAD Antibodies as an Etiology of Cerebral Cortical Encephalitis: 2 Case Reports.
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Introduction: Cerebral cortical encephalitis (CCE) has been recently described as an entity associated with anti-MOG antibodies and, in some cases, of anti-GABAR encephalitis. No clear association with other antibodies has been reported to date. We describe two cases of patients presenting clinical and radiological features of diffuse cortical encephalitis associated with anti-GAD antibodies.
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