sp. (OM1, OM4 and OM7) novel strains were isolated from the gastric fluid of a spotted dolphin (). These strains were phenotypically and genotypically characterized, and compared with known species of the genus . All isolated strains hydrolysed urea and metabolized arginine, but did not produce acid from glucose. All strains were propagated using pleuropneumonia-like organisms medium supplemented with serum and urea under aerobic and anaerobic atmospheric conditions at 37 °C. Transmission electron microscopy revealed a typical mollicute cellular morphology. Analysis of the 16S rRNA gene sequence indicated that the most closely related, validly named type strain was ATCC 43346 (89.4% similarity). The average nucleotide identity and digital DNA-DNA hybridization values among strain OM1 and closely related species were lower than the accepted thresholds for describing novel prokaryotic species at the genomic level. Based on the genomic, phenotypic and phylogenetic properties, the strains represent a novel species of the genus , for which the name sp. nov. with type strain OM1 (=DSM 116106=JCM 39153) is proposed. The genomic G+C content and draft genome sizes of the type strain were 31.7% and 889 711 bp, respectively.
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http://dx.doi.org/10.1099/ijsem.0.006712 | DOI Listing |
J Immunol
January 2025
Institute of Virology and Immunology, Mittelhäusern, Switzerland.
While several African swine fever virus (ASFV)-encoded proteins potently interfere with the cGAS-STING (cyclic GMP-AMP synthetase-stimulator of interferon genes) pathway at different levels to suppress interferon (IFN) type I production in infected macrophages, systemic IFN-α is induced during the early stages of AFSV infection in pigs. The present study elucidates a mechanism by which such responses can be triggered, at least in vitro. We demonstrate that infection of monocyte-derived macrophages (MDMs) by ASFV genotype 2 strains is highly efficient but immunologically silent with respect to IFN type I, IFN-stimulated gene induction, and tumor necrosis factor production.
View Article and Find Full Text PDFElife
March 2025
Department of Biology, Indian Institute of Science Education and Research, Pune, India.
Evolution of gene expression frequently drives antibiotic resistance in bacteria. We had previously (Patel and Matange, , 2021) shown that, in , mutations at the locus were beneficial under trimethoprim exposure and led to overexpression of dihydrofolate reductase (DHFR), encoded by the gene. Here, we show that DHFR levels are further enhanced by spontaneous duplication of a genomic segment encompassing and spanning hundreds of kilobases.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
March 2025
Department of Integrative Biotechnology, Sungkyunkwan University, Natural Science Campus, 2066 Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do 16419, Republic of Korea.
A novel bacterial strain, MJW-29, was isolated from tidal flat sediment in Gochang, Republic of Korea. The isolate is Gram-stain-negative, facultatively anaerobic, gliding motile and short rods. The strain MJW-29 is oxidase and catalase positive.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
March 2025
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Laboratorio de Biotecnología Microbiana. Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, Ciudad de México, Alcaldía Miguel Hidalgo. C.P. 11340, Mexico.
Several and strains from the north of Mexico were isolated during the description of strains. , and were later described as novel species. Next, the Genomic Encyclopedia of Bacteria and Archaea project retrieved the genome sequences of several strains from the above species.
View Article and Find Full Text PDFMicrobiology (Reading)
March 2025
School of Science and Technology, Nottingham Trent University, Nottingham, UK.
Novel treatment options are needed for the gastric pathogen due to its increasing antibiotic resistance. The vitamin K analogue menadione has been extensively studied due to interest in its anti-bacterial and anti-cancer properties. Here, we investigated the effects of menadione on growth, viability, antibiotic resistance, motility and gene expression using clinical isolates.
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