Drug discovery pipelines rely on the availability of isolated primary hepatocytes for investigating potential hepatotoxicity prior to clinical application. These hepatocytes are isolated from livers rejected for transplantation and subsequently cryopreserved for later usage. The gold standard cryopreservation technique, slow-freezing, is a labor-intensive process with significant poststorage viability loss. In this work, we introduce parallelized droplet vitrification, a technique for high-volumetric, rapid vitrification of suspended cells. We show the utility of this technique through the single-run vitrification of the whole rat liver hepatocyte yield, resulting in the vitrification of 250 million cells in 40 mL of a vitrification solution at 10 mL/min. Additionally, we showed that these implementations maintained improved postpreservation outcomes in primary rat hepatocytes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acsami.4c19419 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Parallelized Droplet Vitrification for Single-Run Vitrification of Hepatocytes from an Entire Rat Liver.
ACS Appl Mater Interfaces
March 2025
Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, United States.
Drug discovery pipelines rely on the availability of isolated primary hepatocytes for investigating potential hepatotoxicity prior to clinical application. These hepatocytes are isolated from livers rejected for transplantation and subsequently cryopreserved for later usage. The gold standard cryopreservation technique, slow-freezing, is a labor-intensive process with significant poststorage viability loss.
View Article and Find Full Text PDFCompartmentalization of multiple metabolic enzymes and their preparation in vitro and in cellulo.
Biochim Biophys Acta Gen Subj
March 2025
Department of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Sakai 599-8531, Japan. Electronic address:
Compartmentalization of multiple enzymes in cellulo and in vitro is a means of controlling the cascade reaction of metabolic enzymes. The compartmentation of enzymes through liquid-liquid phase separation may facilitate the reversible control of biocatalytic cascade reactions, thereby reducing the transcriptional and translational burden. This has attracted attention as a potential application in bioproduction.
View Article and Find Full Text PDFComputer vision for high-throughput analysis of pickering emulsions.
Soft Matter
February 2025
Department of Materials Science and Metallurgy, University of Cambridge, CB3 0FS, UK.
The quanitative analysis of solid-particle stabilized emulsions, known as Pickering emulsions, is crucial for their application in food, cosmetics, and pharmaceuticals. However, size analysis of these emulsion droplets, with diameters ranging from 5 to 500 μm, is challenging due to their non-uniform spatial and polydisperse size-distribution. Here, we investigate the application of the circle-Hough transform (CHT), a well-established computer-vision technique characterised by its ability to detect circular features in noisy images, for the seldom explored quantitative assessment of droplet size from optical microscopy images.
View Article and Find Full Text PDFWater Droplet and Its Contact Line Characteristics on Hydrophobic and Hydrophilic Surfaces: A Molecular Dynamics Simulation Approach.
J Phys Chem B
February 2025
Department of Physical Chemistry, School of Chemistry, College of Science, University of Tehran, Tehran 1417935840, Iran.
In literature, for a water droplet on a solid surface, the set of points at the intersection of the three phases, solid_ liquid_ gas, is referred to as the triple phase contact line (TPCL). However, recent studies indicate that the intersections of these phases form a region, which we refer to as the triple phase contact vicinity (TPCV). In the present work, the dimensions of the TPCV, including its width and cross-sectional area, have been calculated for a water droplet on a wide range of hydrophilic and hydrophobic surfaces, using molecular dynamics simulations.
View Article and Find Full Text PDFA digital microfluidic approach to increasing sample volume and reducing bead numbers in single molecule array assays.
Lab Chip
February 2025
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.
We report methods that improve the manipulation of magnetic beads using digital microfluidics (DMF) that can enhance the performance of single molecule array (Simoa) digital protein assays in miniaturized analytical systems. Despite significant clinical and biomedical applications for digital protein detection, the development of miniaturized Simoa systems has been limited by the requirements for use of large sample volumes (∼100 μL) and low numbers of beads (∼5000) for high sensitivity tests. To address these challenges, we improved the integration of DMF with Simoa-based assays by developing strategies for loading mixtures of sample and beads into DMF networks using methods relying on either virtual channels or small liquid segments that were applied either in parallel or in a stepwise manner.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!