Objectives: Infections due to carbapenemase-producing Enterobacterales harboring more than one carbapenemase-encoding gene spreads mainly by plasmid and transposon mobilization.
Objectives: Analyze the mobile genetic elements carrying bla and bla of K. pneumoniae carbapenemase co-producers (KpKN).
Methods: K. pneumoniae isolates with reduced susceptibility to carbapenems were obtained from 2016 to 2023. To evaluate the genetic environment of KpKN, 22 isolates were selected for antimicrobial susceptibility testing and whole-genome sequencing (WGS).
Results: The bla gene was carried mainly by IncN/IncFIB, a novel co-integrated plasmid in the Tn4401b transposon. The bla was disseminated in the only two KpKN isolates recovered before 2020 by IncHI1B/IncFIB plasmid type, in the Tn3000 transposon. Noteworthy, isolates obtained since 2020 showed the bla gene carried by IncA/C in an IS26-flanked pseudo-composite transposon containing ISCR1, which also had genes that confer resistance to sulfonamides, aminoglycosides, macrolides, quinolones, amphenicols, tetracyclines, rifampicin, sulfonamide, and trimethoprim. The isolates belong mainly to ST11 and ST16.
Conclusions: The plasmid and transposon changes during the different periods could be related to higher dissemination of the bla and the large number of resistance genes present in the IS26-flanked transposon may have increased the co-selection of this plasmid through the wide use of antimicrobials during the pandemic.
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