Background: Feeding adult zebrafish a diet supplemented with quenching enzyme AiiO-AIO6 for 3 wk improved the growth performance and disease resistance. However, when the feeding period was extended to 8 wk, zebrafish's disease resistance to Aeromonas veronii decreased.
Objective: We investigated the mechanisms of the reduced disease resistance of zebrafish induced by feeding on AiiO-AIO6 supplemented diet for a long-term (8 wk), and assessed the effectiveness of feed additives in restoring the low disease resistance.
Methods: One-month-old (adult) zebrafish were fed with a basal diet and the basal diet supplemented with AiiO-AIO6 (10 U/g) for 8 wk (Expt. 1). Furthermore, zebrafish larvae model (Expt. 2) was developed and used to study the mechanisms how AiiO-AIO6 affected the disease resistance (Expt. 3). We also investigated the effectiveness of selected prebiotic tributyrin, β-glucan or mannan in activating gut microbiota-hif1α to restore the low disease resistance of adult zebrafish fed with AiiO-AIO6 for 8 wk (Expt. 4). Lastly, the effects of Bacillus subtilis in activating the gut microbiota-hif1α and improving the low disease resistance of zebrafish larvae induced by AiiO-AIO6 was examined (Expt. 5).
Results: Feeding adult zebrafish with AiiO-AIO6 for 8 wk promoted the growth but disordered the gut microbiota and reduced disease resistance. Zebrafish larvae model confirmed that feeding AiiO-AIO6 for 2 d increased disease resistance, while 7 d decreased the resistance by suppressing hif1α. Using a germ-free zebrafish larvae model, we also demonstrated that AiiO-AIO6-induced gut microbiota mediated inhibition of hif1α. Furthermore, zebrafish fed on the AiiO-AIO6 containing diet supplement with tributyrin, β-glucan, mannan, or B. subtilis activated the gut microbiota-hif1α axis to reverse the low resistance caused by AiiO-AIO6.
Conclusion: Activating the gut microbiota-hif1α axis has vital role in improving intestinal health and restores the low resistance to A. veronii caused by improper administration of dietary AiiO-AIO6 in zebrafish.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.tjnut.2025.03.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association between estimated glucose disposal rate and incident cardiovascular disease in a population with Cardiovascular-Kidney-Metabolic syndrome stages 0-3: insights from CHARLS.
Front Cardiovasc Med
February 2025
The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, China.
Objective: Estimated glucose disposal rate (eGDR) is a reliable marker of insulin resistance (IR), which has been proven to be strongly linked to cardiovascular and renal diseases. However, the link between eGDR and the occurrence of cardiovascular disease (CVD) in individuals exhibiting Cardiovascular-Kidney-Metabolic (CKM) syndrome stages 0-3 remains ambiguous.
Methods: The data employed in this investigation was procured from the China Health and Retirement Longitudinal Study (CHARLS).
The long non-coding RNA MSTRG.32189-PcmiR399b- module regulates phosphate accumulation and disease resistance to in pear.
Hortic Res
April 2025
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Pear ring rot disease () is a significant threat to the healthy development of the pear industry. Recent research has identified the functional role of long non-coding RNAs (lncRNAs) in various biological processes of plants. The role of lncRNAs in the pear defense response remains unknown.
View Article and Find Full Text PDFCase Report: A case series on histiocytic sarcoma - various clinical features and patient outcomes.
Front Oncol
February 2025
Department of Hematology, Seoul St. Mary's Hematology Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Introduction: Histiocytic sarcoma (HS) is a rare and aggressive hematologic malignancy with a poor prognosis. HS can present with either isolated organ involvement or multi-systemic disease. This case series reports on nine patients with diverse clinical presentations and outcomes.
View Article and Find Full Text PDFDrug resistance mutations to integrase inhibitors, proteinase, and reverse transcriptase inhibitors in newly diagnosed HIV-1 infections in Hebei province, China, 2018-2022.
Front Cell Infect Microbiol
March 2025
Department of AIDS Research, Hebei Key Laboratory of Pathogen and Epidemiology of Infectious Disease, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang, Hebei, China.
Background: HIV-1 protease (PR)-reverse transcriptase (RT) inhibitors as national free antiretroviral drugs have been used for 20 years. Integrase strand transfer inhibitors (INSTIs) have been conditionally used as a component of HIV/AIDS treatment regimens in recent years. However, the systematic investigation on the changes in primary drug resistance (PDR) in Hebei province, China was limited.
View Article and Find Full Text PDFRevisiting the druggable genome using predicted structures and data mining.
NPJ Drug Discov
March 2025
Department of Pediatrics, University of California, San Diego, La Jolla, CA USA.
Identification of novel drug targets is a key component of modern drug discovery. While antimalarial targets are often identified through the mechanism of action studies on phenotypically derived inhibitors, this method tends to be time- and resource-consuming. The discoverable target space is also constrained by existing compound libraries and phenotypic assay conditions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!