sp. nov., a new species isolated from equine farm soil closely related to the pathogen .

Int J Syst Evol Microbiol

Microbial Pathogenomics Laboratory, Edinburgh Medical School (Biomedical Sciences), University of Edinburgh, Edinburgh, UK.

Published: March 2025

We present the description of the new species, , found during phylogenomic investigations of a global collection of strains identified as () . Strain PAM 2766 was isolated from horse-breeding farm soil in Normandy, France, and was indistinguishable from based on the usual identification tests. Whole-genome phylogenetic analyses located PAM 2766 in the same sublineage as , together with , , , , and . PAM 2766 is most closely related to, but sufficiently distinct from, DSM 20307 to be considered a separate species. The average nt identity (ANI) and average aa identity (AAI) values are 88.60% and 92.35, respectively, well below the species cutoff. The PAM 2766 draft genome is ~5.3 Mb in size with 68.98% G+C mol content. PAM 2766 is strictly aerobic and non-motile and produces smooth, creamy to buff-coloured colonies very similar to those of . It phenotypically differs from the latter by the ability to grow at 5 °C, a strongly positive urease test at 24 h and specificities in the carbon and nitrogen source utilization profile as determined by phenotype microarray screens. Our data indicate that PAM 2766 belongs to a novel species, for which the name sp. nov. is proposed. was avirulent in macrophage infection assays and is assumed to be non-pathogenic. The type strain is PAM 2766 (=CETC 30995=NCTC 14987).

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.006679DOI Listing

Publication Analysis

Top Keywords

pam 2766
28
farm soil
8
strain pam
8
average identity
8
pam
7
0
7
nov species
4
species isolated
4
isolated equine
4
equine farm
4

Similar Publications

sp. nov., a new species isolated from equine farm soil closely related to the pathogen .

Int J Syst Evol Microbiol

March 2025

Microbial Pathogenomics Laboratory, Edinburgh Medical School (Biomedical Sciences), University of Edinburgh, Edinburgh, UK.

We present the description of the new species, , found during phylogenomic investigations of a global collection of strains identified as () . Strain PAM 2766 was isolated from horse-breeding farm soil in Normandy, France, and was indistinguishable from based on the usual identification tests. Whole-genome phylogenetic analyses located PAM 2766 in the same sublineage as , together with , , , , and .

View Article and Find Full Text PDF

High-fidelity CRISPR/Cas12a dual-crRNA screening reveals novel synergistic interactions in hepatocellular carcinoma.

Clin Transl Med

July 2024

State Key Laboratory of Virology, Hubei Key Laboratory of Cell Homeostasis, Department of Biochemistry and Molecular Biology, College of Life Sciences, Wuhan University, Wuhan, China.

Article Synopsis
  • Researchers developed a dual-CRISPR RNA library method for effective combinational genetic screening, aimed at identifying genetic interactions.
  • The method utilizes CeCas12a, which offers precise PAM recognition and minimal off-target effects, enhancing the accuracy of the screening process.
  • By targeting kinase and metabolism-associated genes related to FDA-approved drugs linked to liver cancer, this approach identifies potential genetic interactions that could lead to new cancer treatments.
View Article and Find Full Text PDF

Engineering of a compact, high-fidelity EbCas12a variant that can be packaged with its crRNA into an all-in-one AAV vector delivery system.

PLoS Biol

May 2024

State Key Laboratory of Virology, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Department of Clinical Oncology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.

The CRISPR-associated endonuclease Cas12a has become a powerful genome-editing tool in biomedical research due to its ease of use and low off-targeting. However, the size of Cas12a severely limits clinical applications such as adeno-associated virus (AAV)-based gene therapy. Here, we characterized a novel compact Cas12a ortholog, termed EbCas12a, from the metagenome-assembled genome of a currently unclassified Erysipelotrichia.

View Article and Find Full Text PDF

Engineering of Cas12a nuclease variants with enhanced genome-editing specificity.

PLoS Biol

March 2024

State Key Laboratory of Virology, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Department of Clinical Oncology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a system is a powerful tool in gene editing; however, crRNA-DNA mismatches might induce unwanted cleavage events, especially at the distal end of the PAM. To minimize this limitation, we engineered a hyper fidelity AsCas12a variant carrying the mutations S186A/R301A/T315A/Q1014A/K414A (termed HyperFi-As) by modifying amino acid residues interacting with the target DNA and crRNA strand. HyperFi-As retains on-target activities comparable to wild-type AsCas12a (AsCas12aWT) in human cells.

View Article and Find Full Text PDF

Selected Molecular Mechanisms Involved in the Parasite⁻Host System .

Int J Mol Sci

August 2018

Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wlkp. 72, 70-111 Szczecin, Poland.

The rat tapeworm is a parasite of the small intestine of rodents (mainly mice and rats), and accidentally humans. It is classified as a non-invasive tapeworm due to the lack of hooks on the tapeworm's scolex, which could cause mechanical damage to host tissues. However, many studies have shown that metabolites secreted by interfere with the functioning of the host's gastrointestinal tract, causing an increase in salivary secretion, suppression of gastric acid secretion, and an increase in the trypsin activity in the duodenum chyme.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!