This study explores the molecular mechanism by which sentrin/SUMO-specific protease 1 (SENP1) promotes cisplatin (Cis) resistance and tumor stem cell characteristics in colon adenocarcinoma (COAD) through desumoylation-mediated modification of octamer-binding transcription factor 4 (OCT4). By analyzing single-cell and transcriptome sequencing datasets, we identified key genes and regulatory pathways in both resistant and sensitive COAD cells. Malignant cells were isolated and evaluated for stemness using the infercnv package, and differential genes between Cis-resistant and -sensitive groups were identified. Machine learning algorithms highlighted essential genes, and databases predicted interaction sites between OCT4 and SENP1. experiments using enriched HCT116 stem cells revealed that SENP1 and OCT4 expression significantly elevated CD44 and CD133 levels, enhancing stemness. Functional assays showed that SENP1's desumoylation of OCT4 intensified Cis resistance, migration, and invasion in cisplatin-resistant cell line 116 (Cis-116) cells. , SENP1 knockdown reduced tumor growth and stem cell markers, while OCT4 overexpression escalated tumor metastasis and structural damage. These findings demonstrate that SENP1's modulation of OCT4 is central to COAD's resistance and stem cell properties, offering a novel target for COAD therapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1152/ajpcell.00817.2024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acute kidney injury after lung transplantation, incidence, risk factors, and effects: A Swedish nationwide study.
Acta Anaesthesiol Scand
April 2025
Department of Anesthesiology and Intensive Care Medicine, Institute of Clinical Sciences at the Sahlgrenska Academy, University of Gothenburg and Section for Cardiothoracic Anesthesia and Intensive Care, Sahlgrenska University Hospital, Gothenburg, Sweden.
Background: Acute kidney injury (AKI) is a serious complication after lung transplantation, but the reported incidence varies in the literature. No data on AKI have been published from the Swedish lung transplantation program.
Methods: The aim of our study was to investigate the incidence, perioperative risk factors, and effects of early postoperative acute kidney injury (Kidney Disease Improving Global Outcomes [KDIGO] criteria) after lung transplantation.
Bioactive and degradable collagen-based three-dimensional scaffold encapsulated with adipose mesenchymal stem cells-derived exosomes improved diabetic wound healing.
Regen Ther
March 2025
Cellular and Molecular Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
A diabetic wound is the most frequent form of chronic wound. Because diabetic wounds have multiple factors contributing to their development, the best treatments involve using a combination of approaches. Herein we assessed whether bioactive and degradable bioengineered micro-porous collagen-based three-dimensional scaffold (CTS) encapsulated with adipose mesenchymal stem cells (ASCs)-derived exosomes could accelerate the wound healing process in diabetic rats.
View Article and Find Full Text PDFoncology: a mechanistic multiscale model of clinical prostate cancer response to external radiation therapy as the core of a digital (virtual) twin. Sensitivity analysis and a clinical adaptation approach.
Front Physiol
February 2025
German Cancer Consortium (DKTK), Partner Site Freiburg, Heidelberg, Germany.
Introduction: Prostate cancer (PCa) is the most frequent diagnosed malignancy in male patients in Europe and radiation therapy (RT) is a main treatment option. However, current RT concepts for PCa have an imminent need to be rectified in order to modify the radiotherapeutic strategy by considering (i) the personal PCa biology in terms of radio resistance and (ii) the individual preferences of each patient.
Methods: To this end, a mechanistic multiscale model of prostate tumor response to external radiotherapeutic schemes, based on a discrete entity and discrete event simulation approach has been developed.
Editorial: Current methods for detecting and diagnosing stem cell pathogenesis: a focus on translational interventions in the contemporary disease and technology landscape.
Front Cell Dev Biol
February 2025
Wayne State University, Detroit, MI, United States.
Incidence and Factors Associated with Graft-Versus-Host Disease in the First Year After Allogeneic Peripheral Blood Stem Cell Transplantation.
J Immunother Precis Oncol
May 2025
Department of Internal Medicine, Faculty of Medicine, Pontificia Universidad Javeriana, Hospital Universitario San Ignacio, Bogotá, Colombia.
Introduction: The use of allogeneic peripheral blood stem cell transplantation (allo-SCT) has increased in Latin America in recent years. In the absence of an matched-related donor (MRD), haploidentical transplantation has emerged as a potentially curative option with increasing availability in the region. Graft-versus-host disease (GVHD) is an important complication with variable incidence rates depending on the type of transplant.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!