The reverse transcriptase of Moloney Murine Leukemia Virus (MMLV) is an enzyme that synthesizes DNA from an RNA template. Among reverse transcriptases, this enzyme is currently the most commonly used in molecular biology and diagnostics. Since its discovery, this viral protein has been extensively studied, shedding light on its structural and functional characteristics, and offering opportunities to optimize the catalytic performances for biotechnological applications. The review describes the structural motifs of the reverse transcriptase of MMLV, the key amino acids in enzymatic catalysis and the enzyme's properties such as processivity, fidelity and thermal stability. This review reports the optimizations made to improve these parameters, which enabled the modified enzymes to be integrated in the molecular biology tests used in the laboratory on a daily basis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1684/vir.2025.1070 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of N1-methyladenosine in the quantification of RNA.
J Biochem
March 2025
Department of Pathology, Jinshan Hospital of Fudan University, Shanghai, China.
The reverse transcription (RT) of RNA to cDNA is a key step for the quantification of nucleic acid molecules in numerous basic research and medical diagnosis. Although multiple sources of errors have been considered, little is known about the impact of RNA modifications on the validity of genes of interest for quantitative RT-PCR. Here, we evaluated the influence of RNA modifications of N1-methyladenosine (m1A) on the validity of the RT step by quantifying two RNAs with commercial reverse transcriptase and RNA sample from HEK-293T cells or in vitro transcription.
View Article and Find Full Text PDFA virus-like particle assembly system for probing the HIV-1 Gag-Pol dimerization domain: supporting evidence for reverse transcriptase involvement in protease activation by influencing Gag-Pol/Gag-Pol interaction.
J Virol
March 2025
Institute of Clinical Medicine, National Yang Ming Chiao Tung University-Yangming Campus, Taipei, Taiwan.
Unlabelled: HIV-1 protease (PR) activation is triggered by Gag-Pol dimerization. We previously reported that reverse transcriptase (RT) amino acid substitution mutations resulted in p66/51RT heterodimer instability associated with impaired PR activation, and that treatment with (, an RT dimerization enhancer) increased PR activation, suggesting RT involvement. However, the contribution of RT to PR activation via the promotion of Gag-Pol dimerization has not been corroborated.
View Article and Find Full Text PDFBackground: T-lymphoblastic leukemia (T-ALL) is an aggressive hematologic malignancy with a less favorable prognosis. The genetic background of T-ALL is widely heterogeneous, with the co-occurrence of multiple genetic abnormalities. The STIL-TAL1 rearrangement results from a submicroscopic deletion on chromosome 1p33 and is present in 15 - 25% of T-ALL cases.
View Article and Find Full Text PDFDrug resistance mutations to integrase inhibitors, proteinase, and reverse transcriptase inhibitors in newly diagnosed HIV-1 infections in Hebei province, China, 2018-2022.
Front Cell Infect Microbiol
March 2025
Department of AIDS Research, Hebei Key Laboratory of Pathogen and Epidemiology of Infectious Disease, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang, Hebei, China.
Background: HIV-1 protease (PR)-reverse transcriptase (RT) inhibitors as national free antiretroviral drugs have been used for 20 years. Integrase strand transfer inhibitors (INSTIs) have been conditionally used as a component of HIV/AIDS treatment regimens in recent years. However, the systematic investigation on the changes in primary drug resistance (PDR) in Hebei province, China was limited.
View Article and Find Full Text PDF[MMLV reverse transcriptase, a relevant tool for molecular biology].
Virologie (Montrouge)
February 2025
Unité des Virus émergents (UVE : Aix-Marseille Univ, Università di Corsica, Corte, IRD 190, Inserm 1207, IRBA), France.
The reverse transcriptase of Moloney Murine Leukemia Virus (MMLV) is an enzyme that synthesizes DNA from an RNA template. Among reverse transcriptases, this enzyme is currently the most commonly used in molecular biology and diagnostics. Since its discovery, this viral protein has been extensively studied, shedding light on its structural and functional characteristics, and offering opportunities to optimize the catalytic performances for biotechnological applications.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!