Glutathione S-transferase polymorphisms and their role in recurrent pregnancy loss: A genetic risk assessment.

Objective: The frequency of recurrent pregnancy loss in society is 3-5%. Experts suggest that genetics account for over 80% of unexplained recurrent pregnancy loss. Glutathione S-transferase (GST) enzymes, regulated by GST genes, facilitate the detoxification of a variety of naturally occurring metabolites as well as environmentally derived chemicals. This research aimed to investigate GST gene polymorphisms as a potential risk factor in recurrent pregnancy loss etiology in the Turkish population.

Materials And Methods: This study involved 107 recurrent pregnancy loss patients who sought treatment at the Sivas Cumhuriyet University Faculty of Medicine, Department of Medical Genetics, along with a control group of 107 individuals who had a successful birth and no previous history of miscarriage. The multiplex polymerase chain reaction and restriction fragment length polymorphism techniques were employed to analyze and gene polymorphisms in these cases.

Results: GSTT1 null genotype (X=4.74; p=0.029) and GSTT1/GSM1 null genotype (X=3.333; p=0.047) were associated with statistically significant differences between the study groups. No statistical significance was detected when considering the GSTM1 null genotype (X=3.326; p=0.068) or the and gene polymorphisms.

Conclusion: A statistically significant association was observed between the GSTT1 null genotype and the diseased group. Our research demonstrated a substantial increase in the risk of recurrent pregnancy loss in the Turkish population, specifically among individuals with the GSTM1-null genotype. No statistical correlation was found between the and gene polymorphisms and recurrent pregnancy loss. Furthermore, no statistical significance was observed when they were assessed together.