In recent years, much research has focused on the valorization of Crocus sativus by-products as sources of bioactive compounds. This study was designed to investigate the impact of two different drying methods, freeze-drying and air-drying, on the phenolic content and antioxidant activity of leaves and tepals by-products from C. sativus collected in Meknes, Morocco. Stigmas were included in the study to provide further information on this precious spice. The phenolic content of the hydroalcoholic extracts was determined spectrophotometrically and characterized by HPLC-PDA/ESI-MS analysis. The antioxidant properties were evaluated by different in vitro assays. Both in the DPPH and in the ferrous ion chelating activity assays the extracts from freeze-dried tepals (Cs-TFD) and leaves (Cs-LFD) displayed higher activity than those from air-dried plant materials. Cs-LFD showed the best scavenging activity, while superimposable chelating activity was observed for Cs-TFD and Cs-LFD. Finally, no toxicity was observed in the Artemia salina lethality bioassay.
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