Investigation of Antimicrobial and Antioxidant Properties of (Ehrenb.) Bunge Leaf Extract Against Catheter-Associated Urinary Tract Infections (CAUTIs) in Al Kharj Governorate.

Introduction: One of most important nosocomial infections, Catheter associated urinary tract infections (CAUTI) are caused by many biofilms forming organism which makes treatment challenges as well as resistant strain development. To prevent the CAUTI, the new antimicrobials are needed immediately.

Methodology: The overnight grown cultures such as , , and were grown in Brain Heart Infusion (BHI) broth, Mueller Hinton Broth (MHB), and Sabouraud Dextrose Broth (SDB). Ampicillin, rifampicin, and nystatin were used in all the experiments as positive controls and also, methanol was used as vehicle control.

Results: The leaf extract showed antimicrobial activity at 2 mg/ml against and 1 mg/ml against and . Further, crystal violet staining revealed, antibiofilm activity through biofilm inhibition and also, it was efficiently eliminated 77%, 73%, and 78% of , and mature biofilms individually. Later, catheter coating with leaf extract explored strong antimicrobial activity against tested pathogens in in vitro bladder model. In addition, leaf extract expressed strong antioxidant property.

Conclusion: can be considered as alternative antimicrobial agent for CAUTI treatment.