Dominance of Vaccine-Specific Genotypes in Koalas From North-Eastern Australia.

is a significant cause of morbidity and mortality in koalas and a major contributor to population decline. Due to its crucial role in vaccine development and use as a strain typing tool, multiple studies have investigated the prevalence and diversity of the outer membrane protein A (MOMP), encoded by A. This prior work has shown that A genotypes vary across geographical regions, with multiple genotypes identified across Eastern Australia. Despite these earlier studies, our understanding of the diversity and distribution of MOMP remains incomplete. Here, we aimed to assess the geographical distribution, diversity, and temporal stability of the A across wild koalas infected with in north-eastern Australia. Full-length A sequences were generated from 226 samples collected from 173 unique animals from multiple koala populations over a 4-year time span. A genotypes F and G were the most common and were identified across all geographical regions. Genotype A was the next most common but mostly restricted to the South Brisbane region. In addition to two novel genotypes, rare genotypes, including livestock-associated types E58 and L1, and koala-associated H and I were sporadically identified. Comparison with historical A genotypes showed that the distribution of genotypes has remained stable over the past decade, suggesting limited selective pressure despite ongoing vaccination trials and management programmes. The stability of genotypes coupled with the identification of novel and livestock-associated genotypes underscores the importance of continued surveillance to inform future vaccine development and conservation strategies for koalas.