Severity: Warning
Message: fopen(/var/lib/php/sessions/ci_sessionlc9cu1jaida9i03ld06djqlde3gras5d): Failed to open stream: No space left on device
Filename: drivers/Session_files_driver.php
Line Number: 177
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Severity: Warning
Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)
Filename: Session/Session.php
Line Number: 137
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Severity: Warning
Message: file_get_contents(https://...@remsenmedia.com&api_key=81853a771c3a3a2c6b2553a65bc33b056f08&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 197
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 197
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 271
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3145
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
The use of genetically engineered tools, including combinations of Cre-LoxP and Flp-FRT systems, enable the interrogation of complex biology. Steroidogenic factor-1 (SF-1) is expressed in the ventromedial hypothalamic nucleus (VMH). Development of genetic tools, such as mice expressing Flp recombinase (Flp) in SF-1 neurons ( -Flp), will be useful for future studies that unravel the complex physiology regulated by the VMH. Here, we developed and characterized -Flp mice and demonstrated its utility. Flp sequence was inserted into locus with P2A. This insertion did not affect mRNA expression levels and -Flp mice do not have any visible phenotypes. They are fertile and metabolically comparable to wild-type littermate mice. Optogenetic stimulation using adeno-associated virus (AAV)-bearing Flp-dependent channelrhodopsin-2 (ChR2) increased blood glucose and skeletal muscle PGC-1α in -Flp mice. This was similar to SF-1 neuronal activation using -BAC-Cre and AAV-bearing Cre-dependent ChR2. Finally, we generated -Flp mice that lack ( ) only in skeletal muscle with a combination of Cre/LoxP technology ( -Flp::SKM ). Optogenetic stimulation of SF-1 neurons failed to increase skeletal muscle PGC-1α in -Flp::SKM mice, suggesting that in skeletal muscle is for augmented skeletal muscle PGC-1α by SF-1 neuronal activation. Our data demonstrate that -Flp mice are useful for interrogating complex physiology.
Download full-text PDF |
Source |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11888304 | PMC |
http://dx.doi.org/10.1101/2025.02.21.639566 | DOI Listing |
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